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PXD046785

PXD046785 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTOPOVIBL∆C25 mouse protein purification from insect cells
DescriptionThe project aims at studying in vitro the TOPOVIBL∆C25 protein. This protein is essential to the formation of DNA double strand breaks, which initiates homologous recombination in meiosis in the mice. We set up a purification procedure of the recombinant TOPOVIBL∆C25 protein expressed in insect cells, which was validated using MS/MS approaches.
HostingRepositoryPRIDE
AnnounceDate2024-07-09
AnnouncementXMLSubmission_2024-07-08_23:29:51.334.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD046785
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterFunctional Proteomics Platform FPP
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-11-08 07:26:50ID requested
12024-07-08 23:29:52announced
Publication List
Diagouraga B, Tambones I, Carivenc C, Bechara C, Nadal M, de Massy B, le Maire A, Robert T, The TOPOVIBL meiotic DSB formation protein: new insights from its biochemical and structural characterization. Nucleic Acids Res, 52(15):8930-8946(2024) [pubmed]
10.6019/PXD046785;
10.1093/nar/gkae587;
Keyword List
submitter keyword: MS/MS,Mouse, Insect cells, testes
Contact List
Thomas Robert
contact affiliationCentre de Biologie Structurale (CBS), CNRS, INSERM, Univ Montpellier, 34090 Montpellier, France
contact emailthomas.robert@cbs.cnrs.fr
lab head
Functional Proteomics Platform FPP
contact affiliationFPP CNRS
contact emailcontact@fpp.cnrs.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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