PXD046777

PXD046777 is an original dataset announced via ProteomeXchange.

Title	Direct translation of incoming retroviral genomes
Description	Viruses that carry a positive-sense, single-stranded (+ssRNA) RNA translate their genomes soon after entering the host cell to produce viral proteins, with the exception of retroviruses. A distinguishing feature of retroviruses is reverse transcription, where the +ssRNA genome serves as a template to synthesize a double-stranded DNA copy that subsequently integrates into the host genome. As retroviral RNAs are produced by the host cell transcriptional machinery and are largely indistinguishable from cellular mRNAs, we investigated the potential of incoming retroviral genomes to directly express proteins. Here we show through multiple, complementary methods that retroviral genomes are translated after entry. Our findings challenge the notion that retroviruses require reverse transcription to produce viral proteins. Synthesis of retroviral proteins in the absence of productive infection has significant implications for basic retrovirology, immune responses and gene therapy applications.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_07:30:16.627.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joerg Doellinger
SpeciesList	scientific name: Human immunodeficiency virus 1; NCBI TaxID: 11676; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Bruker Daltonics timsTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-08 02:54:25	ID requested
⏵ 1	2024-01-26 07:30:17	announced

10.1038/s41467-023-44501-7;

K, ö, ppke J, Keller LE, Stuck M, Arnow ND, Bannert N, Doellinger J, Cing, ö, z O, Direct translation of incoming retroviral genomes. Nat Commun, 15(1):299(2024) [pubmed]

submitter keyword: HIV, SILAC, IP-MS

Peter Lasch
contact affiliation	ZBS6, Proteomics and Spectroscopy, Robert Koch-Institute
contact email	LaschP@rki.de
lab head
Joerg Doellinger
contact affiliation	Proteomics and Spectroscopy (ZBS6), Robert Koch-Institute
contact email	Doellingerj@rki.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD046777

PRIDE project URI

• PRIDE
  1. PXD046777
     1. Label: PRIDE project
     2. Name: Direct translation of incoming retroviral genomes