PXD046742

PXD046742 is an original dataset announced via ProteomeXchange.

Title	SUZ12 immunoprecipitation followed by PRM of SUZ12 peptides to identify alternatively spliced isoforms of SUZ12 at the protein level.
Description	Alternative splicing of SUZ12 exon 4 is predicted to generate two alternative isoforms differing by the inclusion or exclusion of 69 nucleotides in exon 4, which we named SUZ12-long (SUZ12-L) and SUZ12-short (SUZ12-S) respectively. At the protein level SUZ12 exon 4 encodes 23 amino acids (aa 129–152 in SUZ12-L) that partially overlap with the WD-binding domain 1 (WDB1, 110–145). To detect the existance of SUZ12-S at the protein levels we performed SUZ12 immunoprecipitation coupled with mass spectrometry (IP-MS) in the WT and ∆ex4 clones to identify SUZ12-S unique peptides. We identified a SUZ12-S specific peptide in both WT #2 and ∆ex4 #1. These observations were confirmed by SUZ12 IP followed by Western blot (WB) (size shift).
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:33:51.772.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Niccolò Arecco
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-07 06:46:47	ID requested
1	2024-03-18 03:44:48	announced
⏵ 2	2024-10-22 06:33:52	announced	2024-10-22: Updated project metadata.

10.1016/j.molcel.2024.02.011;

Arecco N, Mocavini I, Blanco E, Ballar, é C, Libman E, Bonnal S, Irimia M, Di Croce L, Alternative splicing decouples local from global PRC2 activity. Mol Cell, 84(6):1049-1061.e8(2024) [pubmed]

submitter keyword: exon, AS, IP, PRC2,mESCs, alternative splicing, PRM., ESCs, SUZ12, Polycomb

Niccolò Arecco
contact affiliation	Centre for Genomic Regulation (CRG), Barcelona, Spain
contact email	arecco.niccolo@gmail.com
lab head
Niccolò Arecco
contact affiliation	CRG
contact email	arecco.niccolo@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/03/PXD046742

PRIDE project URI

• PRIDE
  1. PXD046742
     1. Label: PRIDE project
     2. Name: SUZ12 immunoprecipitation followed by PRM of SUZ12 peptides to identify alternatively spliced isoforms of SUZ12 at the protein level.