RNF12/RLIM is an E3 ubiquitin ligase that plays key developmental roles and is genetically disrupted in the neurodevelopmental disorder Tonne-Kalscheuer syndrome. RNF12/RLIM regulates X-chromosome inactivation and controls the expression of neural and gametogenesis related genes. To better understand how RNF12/RLIM performs these transcription-related functions, we performed a TurboID proximity ligation experiment to identify RNF12/RLIM proximal proteins in mouse embryonic stem cells treated with DMSO or MG132. This knowledge will increase our understanding of how this protein controls developmental gene expression and how disruption of these pathways drives disease.