PXD046710 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | RNA helicase EIF4A1-mediated translation is essential for the GC response |
Description | EIF4A1 and cofactors EIF4B and EIF4H have been well characterised in cancers, including B cell malignancies, for their ability to promote the translation of oncogenes with structured 5’ untranslated regions. However, very little is known of their roles in non-malignant cells. Using mouse models to delete Eif4a1, Eif4b or Eif4h in B cells we show that EIF4A1, but not EIF4B or EIF4H, is essential for B cell development and the germinal centre response. Following B cell activation in vitro, EIF4A1 facilitates an increased rate of protein synthesis, MYC expression and expression of cell cycle regulators. However, EIF4A1-deficient cells remain viable whereas inhibition of EIF4A1 and EIF4A2 by Hippuristanol treatment induces cell death. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:13:53.788.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Andrew Howden |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-11-06 13:49:25 | ID requested | |
1 | 2023-11-09 12:56:57 | announced | |
⏵ 2 | 2024-10-22 06:13:54 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: EIF4A1, B cells, translation |
Contact List
Professor Martin Turner |
contact affiliation | Immunology Programme, The Babraham Institute, Babraham Research Campus, Cambridge, CB22 3AT, United Kingdom. |
contact email | martin.turner@babraham.ac.uk |
lab head | |
Andrew Howden |
contact affiliation | University of Dundee |
contact email | a.howden@dundee.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD046710
- Label: PRIDE project
- Name: RNA helicase EIF4A1-mediated translation is essential for the GC response