PXD046710

PXD046710 is an original dataset announced via ProteomeXchange.

Title	RNA helicase EIF4A1-mediated translation is essential for the GC response
Description	EIF4A1 and cofactors EIF4B and EIF4H have been well characterised in cancers, including B cell malignancies, for their ability to promote the translation of oncogenes with structured 5’ untranslated regions. However, very little is known of their roles in non-malignant cells. Using mouse models to delete Eif4a1, Eif4b or Eif4h in B cells we show that EIF4A1, but not EIF4B or EIF4H, is essential for B cell development and the germinal centre response. Following B cell activation in vitro, EIF4A1 facilitates an increased rate of protein synthesis, MYC expression and expression of cell cycle regulators. However, EIF4A1-deficient cells remain viable whereas inhibition of EIF4A1 and EIF4A2 by Hippuristanol treatment induces cell death.
HostingRepository	PRIDE
AnnounceDate	2023-11-09
AnnouncementXML	Submission_2023-11-09_12:56:57.003.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	AndrewHowden
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-06 13:49:25	ID requested
⏵ 1	2023-11-09 12:56:57	announced

Dataset with its publication pending

submitter keyword: EIF4A1, B cells, translation

Professor MartinTurner
contact affiliation	Immunology Programme, The Babraham Institute, Babraham Research Campus, Cambridge, CB22 3AT, United Kingdom.
contact email	martin.turner@babraham.ac.uk
lab head
AndrewHowden
contact affiliation	University of Dundee
contact email	a.howden@dundee.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046710
     1. Label: PRIDE project
     2. Name: RNA helicase EIF4A1-mediated translation is essential for the GC response