PXD046710

PXD046710 is an original dataset announced via ProteomeXchange.

Title	RNA helicase EIF4A1-mediated translation is essential for the GC response
Description	EIF4A1 and cofactors EIF4B and EIF4H have been well characterised in cancers, including B cell malignancies, for their ability to promote the translation of oncogenes with structured 5’ untranslated regions. However, very little is known of their roles in non-malignant cells. Using mouse models to delete Eif4a1, Eif4b or Eif4h in B cells we show that EIF4A1, but not EIF4B or EIF4H, is essential for B cell development and the germinal centre response. Following B cell activation in vitro, EIF4A1 facilitates an increased rate of protein synthesis, MYC expression and expression of cell cycle regulators. However, EIF4A1-deficient cells remain viable whereas inhibition of EIF4A1 and EIF4A2 by Hippuristanol treatment induces cell death.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:13:53.788.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrew Howden
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-06 13:49:25	ID requested
1	2023-11-09 12:56:57	announced
⏵ 2	2024-10-22 06:13:54	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: EIF4A1, B cells, translation

Professor Martin Turner
contact affiliation	Immunology Programme, The Babraham Institute, Babraham Research Campus, Cambridge, CB22 3AT, United Kingdom.
contact email	martin.turner@babraham.ac.uk
lab head
Andrew Howden
contact affiliation	University of Dundee
contact email	a.howden@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD046710

PRIDE project URI

• PRIDE
  1. PXD046710
     1. Label: PRIDE project
     2. Name: RNA helicase EIF4A1-mediated translation is essential for the GC response