PXD046708

PXD046708 is an original dataset announced via ProteomeXchange.

Title	SART3 is a Novel Rme2s Reader studied by tandem MS
Description	Arginine methylation is a ubiquitous post-translational modification involved in many biological processes such as transcription, cell signaling and RNA splicing. Tudor domains by far are the major effector domain family for arginine methylation mark recognition. Here we characterize SART3 as a novel selective reader for symmetric dimethylarginine (Rme2s) marks. SART3 harbors a series of HAT (Half-a-TPR) repeats that are aromatic-rich, and it is this region that binds Rme2s motifs. An analysis of the reported structure of the HAT repeats of SART3 identified a putative aromatic cage that potentially “read” the Rme2s-modified motifs, and the key aromatic residues required for Rme2s recognition were identified. We further confirm that this Rme2s reader ability is important for SART3-mediated RNA splicing. Together, our studies revealed that the evolutionarily conserved SART3 HAT domain was a novel reader of Rme2s mark involved in RNA splicing.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_02:38:53.882.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD046708
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	William Russell
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monomethylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-06 13:07:33	ID requested
⏵ 1	2024-10-17 02:38:54	announced

10.6019/PXD046708;

10.1016/j.celrep.2024.114459;

Wang Y, Zhou J, He W, Fu R, Shi L, Dang NK, Liu B, Xu H, Cheng X, Bedford MT, SART3 reads methylarginine-marked glycine- and arginine-rich motifs. Cell Rep, 43(7):114459(2024) [pubmed]

submitter keyword: apms, lcms,Arginine methylation

Mark Bedford
contact affiliation	UT-MDACC
contact email	mtbedford@mdanderson.org
lab head
William Russell
contact affiliation	UT-Medical Branch
contact email	bill.russell@utmb.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046708
     1. Label: PRIDE project
     2. Name: SART3 is a Novel Rme2s Reader studied by tandem MS