PXD046704 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Two paralogous PHD finger proteins participate in Paramecium tetraurelia's natural genome editing |
Description | The unicellular eukaryote Paramecium tetraurelia contains functionally distinct nuclei: germline micronuclei (MICs) and a somatic macronucleus (MAC). During sexual reproduction, the MIC genome is reorganized into a new MAC genome and the old MAC is lost. Almost 45,000 unique Internal Eliminated Sequences (IESs) located throughout the genome require precise excision to guarantee a functional new MAC genome. Here, we characterize a pair of paralogous PHD finger proteins involved in DNA elimination. DevPF1, the early-expressed paralog, is present in only some of the gametic and post-zygotic nuclei during meiosis. Both DevPF1 and DevPF2 localize in the new developing MACs, where IESs excision occurs. In DevPF2 knockdown (KD), long IESs are preferentially retained and late-expressed small RNAs decrease; no length preference for retained IESs was observed in DevPF1-KD and development-specific small RNAs were abolished. The expression of at least two genes from the new MAC seems to be influenced by DevPF1- and DevPF2-KD. Thus, both PHD fingers are crucial for new MAC genome development, with distinct functionalities, potentially via regulation of non-coding and coding transcription in the MICs and new MACs. |
HostingRepository | PRIDE |
AnnounceDate | 2024-08-30 |
AnnouncementXML | Submission_2024-08-30_06:17:35.809.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Lilia Häußermann |
SpeciesList | scientific name: Paramecium tetraurelia; NCBI TaxID: 5888; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-11-06 08:03:00 | ID requested | |
⏵ 1 | 2024-08-30 06:17:36 | announced | |
Publication List
Keyword List
submitter keyword: PHD finger proteins |
co-IP |
Contact List
Estienne Swart |
contact affiliation | Max-Planck-Institute for Biology |
contact email | estienne.swart@tuebingen.mpg.de |
lab head | |
Lilia Häußermann |
contact affiliation | Max-Planck-Society |
contact email | lilia.haeussermann@tuebingen.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD046704
- Label: PRIDE project
- Name: Two paralogous PHD finger proteins participate in Paramecium tetraurelia's natural genome editing