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PXD046704

PXD046704 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTwo paralogous PHD finger proteins participate in Paramecium tetraurelia's natural genome editing
DescriptionThe unicellular eukaryote Paramecium tetraurelia contains functionally distinct nuclei: germline micronuclei (MICs) and a somatic macronucleus (MAC). During sexual reproduction, the MIC genome is reorganized into a new MAC genome and the old MAC is lost. Almost 45,000 unique Internal Eliminated Sequences (IESs) located throughout the genome require precise excision to guarantee a functional new MAC genome. Here, we characterize a pair of paralogous PHD finger proteins involved in DNA elimination. DevPF1, the early-expressed paralog, is present in only some of the gametic and post-zygotic nuclei during meiosis. Both DevPF1 and DevPF2 localize in the new developing MACs, where IESs excision occurs. In DevPF2 knockdown (KD), long IESs are preferentially retained and late-expressed small RNAs decrease; no length preference for retained IESs was observed in DevPF1-KD and development-specific small RNAs were abolished. The expression of at least two genes from the new MAC seems to be influenced by DevPF1- and DevPF2-KD. Thus, both PHD fingers are crucial for new MAC genome development, with distinct functionalities, potentially via regulation of non-coding and coding transcription in the MICs and new MACs.
HostingRepositoryPRIDE
AnnounceDate2024-08-30
AnnouncementXMLSubmission_2024-08-30_06:17:35.809.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLilia Häußermann
SpeciesList scientific name: Paramecium tetraurelia; NCBI TaxID: 5888;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-11-06 08:03:00ID requested
12024-08-30 06:17:36announced
Publication List
10.1101/2024.01.23.576875;
Keyword List
submitter keyword: PHD finger proteins
co-IP
Contact List
Estienne Swart
contact affiliationMax-Planck-Institute for Biology
contact emailestienne.swart@tuebingen.mpg.de
lab head
Lilia Häußermann
contact affiliationMax-Planck-Society
contact emaillilia.haeussermann@tuebingen.mpg.de
dataset submitter
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Dataset FTP location
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