PXD046704

PXD046704 is an original dataset announced via ProteomeXchange.

Title	Two paralogous PHD finger proteins participate in Paramecium tetraurelia's natural genome editing
Description	The unicellular eukaryote Paramecium tetraurelia contains functionally distinct nuclei: germline micronuclei (MICs) and a somatic macronucleus (MAC). During sexual reproduction, the MIC genome is reorganized into a new MAC genome and the old MAC is lost. Almost 45,000 unique Internal Eliminated Sequences (IESs) located throughout the genome require precise excision to guarantee a functional new MAC genome. Here, we characterize a pair of paralogous PHD finger proteins involved in DNA elimination. DevPF1, the early-expressed paralog, is present in only some of the gametic and post-zygotic nuclei during meiosis. Both DevPF1 and DevPF2 localize in the new developing MACs, where IESs excision occurs. In DevPF2 knockdown (KD), long IESs are preferentially retained and late-expressed small RNAs decrease; no length preference for retained IESs was observed in DevPF1-KD and development-specific small RNAs were abolished. The expression of at least two genes from the new MAC seems to be influenced by DevPF1- and DevPF2-KD. Thus, both PHD fingers are crucial for new MAC genome development, with distinct functionalities, potentially via regulation of non-coding and coding transcription in the MICs and new MACs.
HostingRepository	PRIDE
AnnounceDate	2024-08-30
AnnouncementXML	Submission_2024-08-30_06:17:35.809.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lilia Häußermann
SpeciesList	scientific name: Paramecium tetraurelia; NCBI TaxID: 5888;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-06 08:03:00	ID requested
⏵ 1	2024-08-30 06:17:36	announced

10.1101/2024.01.23.576875;

submitter keyword: PHD finger proteins

co-IP

Estienne Swart
contact affiliation	Max-Planck-Institute for Biology
contact email	estienne.swart@tuebingen.mpg.de
lab head
Lilia Häußermann
contact affiliation	Max-Planck-Society
contact email	lilia.haeussermann@tuebingen.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046704
     1. Label: PRIDE project
     2. Name: Two paralogous PHD finger proteins participate in Paramecium tetraurelia's natural genome editing