PXD046697

PXD046697 is an original dataset announced via ProteomeXchange.

Title	The cell cycle controls spindle architecture in Arabidopsis by modulating the augmin pathway
Description	To ensure an even segregation of chromosomes during somatic cell division, eukaryotes rely on specific microtubule structures called mitotic spindles. There are however striking differences in overall spindle organization among eukaryotic super groups, and in particular little is known about how spindle architecture is determined in plants. As a foundation for our work, we have measured prime characteristics of Arabidopsis mitotic spindles and built a three-dimensional dynamic model of the Arabidopsis mitotic spindle using Cytosim. Next, we identified the cell-cycle regulator CYCLIN-DEPENDENT KINASE B1 (CDKB1) together with its cyclin partner CYCB3;1 as key regulators of spindle shape and organization in Arabidopsis. Loss of CDKB1 function resulted in a high number of astral microtubules that are normally absent from plant spindles, as opposed to animal ones. We identified an augmin complex member, ENDOSPERM DEFECTIVE1 (EDE1), as a substrate of the CDKB1;1-CYCB3;1 complex. A non-phosphorylatable mutant of EDE1 displayed spindles with extended pole-to-pole distance, resembling the phenotypes of cycb3;1 and cdkb1 mutants, and the mutated EDE1 associated less efficiently with spindle microtubules. Consistently, reducing the level of augmin in Cytosim simulations largely recapitulated the spindle phenotypes observed in cycb3;1 and cdkb1 mutants. Our results emphasize the importance of cell cycle-dependent phospho-control of the mitotic spindle in plant cells. They also support the validity of our computational model as a framework for the exploration of mechanisms controlling the organization of plant spindles
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:56:36.683.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sara Christina Stolze
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-06 04:03:19	ID requested
1	2024-08-26 23:52:58	announced
⏵ 2	2024-10-22 06:56:37	announced	2024-10-22: Updated project metadata.

10.1016/J.DEVCEL.2024.08.001;

submitter keyword: augmin, plant biology,Spindle, cell division, mitosis, phosphorylation, cyclin, cell cycle, cyclin-dependent kinase, developmental biology

Hirofumi Nakagami
contact affiliation	Basic Immune System of Plants / Protein Mass Spectrometry Max Planck Institute for Plant Breeding Research Carl-von-Linne-Weg 10, 50829 Cologne, Germany
contact email	nakagami@mpipz.mpg.de
lab head
Sara Christina Stolze
contact affiliation	Plant Proteomics and Mass Spectrometry Group Max Planck Institute for Plant Breeding Research Carl-von-Linné Weg 10 50829 Cologne
contact email	stolze@mpipz.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046697
     1. Label: PRIDE project
     2. Name: The cell cycle controls spindle architecture in Arabidopsis by modulating the augmin pathway