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PXD046691

PXD046691 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation
DescriptionThe formation of new ribosomes is tightly coordinated with cell growth and proliferation. In eukaryotes, the correct assembly of all ribosomal proteins and RNAs involves an intricate cascade of maturation and rearrangement steps across three cellular compartments: the nucleolus, nucleoplasm, and cytoplasm. We demonstrate that usnic acid, a lichen secondary metabolite, inhibits the maturation of the large ribosomal subunit in yeast. We combined biochemical characterization of pre-ribosomal particles with a quantitative single-particle cryo-EM approach to monitor changes in nucleolar particle populations upon drug treatment. Usnic acid rapidly blocks the transition from nucleolar state B to C of Nsa1-associated pre-ribosomes, depleting key maturation factors such as Dbp10 and hindering pre-rRNA processing. This primary nucleolar block rapidly rebounds on earlier stages of the pathway highlighting regulatory linkages between different maturation stages. In summary, we provide an in-depth characterization of the effect of usnic acid on ribosome biogenesis, which may have implications for its reported anti-cancer activities.
HostingRepositoryPRIDE
AnnounceDate2024-10-17
AnnouncementXMLSubmission_2024-10-17_03:58:19.870.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJuliane Merl-Pham
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListmonohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF; Q Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-11-06 00:43:34ID requested
12024-10-17 03:58:20announced
Publication List
10.1038/s41467-024-51754-3;
Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H, The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation. Nat Commun, 15(1):7511(2024) [pubmed]
Keyword List
submitter keyword: yeast, 60s, ribosome biogenesis,LC-MSMS
Contact List
Helmut Bergler
contact affiliationInstitute of Molecular Biosciences, University of Graz, Graz, 8010, Austria
contact emailhelmut.bergler@uni-graz.at
lab head
Juliane Merl-Pham
contact affiliationMetabolomics and Proteomics Core, Helmholtz Munich
contact emailjuliane.merl@helmholtz-muenchen.de
dataset submitter
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