PXD046688

PXD046688 is an original dataset announced via ProteomeXchange.

Title	Jie-Du-Tong-Luo formula protects C2C12 myotubes against high glucose and palmitic acid injury by activating the PI3K/Akt/PPARγ pathway in vitr
Description	Introduction: In prior reports, Jie-Du-Tong-Luo (JDTL) was reported to help control insulin secretion and blood glucose in patients with diabetes, while also protecting liver and pancreatic islet cells against injury caused by exposure to high glucose (HG) levels. This study was thus developed to assess the effects of JDTL on HG and palmitic acid (PA)-induced muscle injury and to explore the mechanistic basis for these effects. Methods: A model of muscle injury was established using mouse C2C12 myotubes treated with HG+PA. A proteomics approach was used to assess changes in protein levels following JDTL treatment, after which Western immunoblotting was employed to validate significantly affected pathways. Results: JDTL was able to protect against HG+PA-induced muscle cell injury in this experimental system, altering lipid metabolism and inflammatory activity in these injured C2C12 myotubes. Western blotting suggested that JDTL is capable of activating PI3K/Akt/PPARγ signaling to control lipid metabolism without any corresponding impact on the inflammatory NF-κB pathway. Conclusions: These data highlight the ability of JDTL to protect against HG+PA-induced injury to muscle cells, and suggest that the underlying basis for such efficacy is related to the PI3K/Akt/PPARγ pathway-mediated modulation of lipid metabolism.
HostingRepository	iProX
AnnounceDate	2023-11-04
AnnouncementXML	Submission_2023-11-05_19:00:33.802.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manying Wang
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-05 19:00:19	ID requested
⏵ 1	2023-11-05 19:00:34	announced

Dataset with its publication pending

submitter keyword: Jie-Du-Tong-Luo formula, high glucose, palmitic acid, C2C12 myotubes, PI3K/Akt/PPARγ

Manying Wang
contact affiliation	Affiliated Hospital to Changchun University of Chinese Medicine
contact email	851721642@qq.com
lab head
Manying Wang
contact affiliation	Affiliated Hospital to Changchun University of Chinese Medicine
contact email	851721642@qq.com
dataset submitter

iProX dataset URI