PXD046672 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis of Mason-Pfizer monkey virus virions |
Description | To accomplish their replication cycle, retroviruses rely heavily on a wide variety of cellular proteins and enzymes, such as helicases. Among these, the DExD-box and DEAH/RHA-box helicases were identified to play crucial roles in their nucleic acid metabolism. This study explores the Betaretrovirus Mason-Pfizer monkey virus (M-PMV), which uniquely encodes a G-patch motif - a feature shared with several endogenous retroviruses. Typically found in eukaryotic RNA-binding proteins, G-patch acts as cofactors for DEAH-box RNA helicases, guiding them to specific sub-cellular sites and activating them in diverse RNA metabolic pathways. Despite its established importance in the betaretrovirus replication cycle, the function of the G-patch still needs to be discovered. Using MS analysis, we identified DHX15, a cellular DEAH-box RNA helicase, as host cell protein macked into mature virions. Further research illuminates the complex interplay between retroviruses and host cellular machinery, highlighting the pivotal role of helicases in viral replication processes. |
HostingRepository | PRIDE |
AnnounceDate | 2024-09-13 |
AnnouncementXML | Submission_2024-09-13_02:34:42.912.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Petra Junková |
SpeciesList | scientific name: Mason-Pfizer monkey virus; NCBI TaxID: 11855; |
ModificationList | No PTMs are included in the dataset |
Instrument | maXis |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-11-05 01:12:09 | ID requested | |
⏵ 1 | 2024-09-13 02:34:43 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Mason-Pfizer monkey virus, DHX15, G-patch domain, HEK 293, LC-MS/MS |
Contact List
Michaela Rumlová |
contact affiliation | Department of Biotechnology, University of Chemistry and Technology, Prague, 166 28 Prague, Czech Republic |
contact email | rumlovai@vscht.cz |
lab head | |
Petra Junková |
contact affiliation | Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo nám. 2, 166 10 Prague |
contact email | petra.junkova@uochb.cas.cz |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD046672 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD046672
- Label: PRIDE project
- Name: Proteomic analysis of Mason-Pfizer monkey virus virions