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PXD046669

PXD046669 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSimultaneous Proteome Localization and Turnover Analysis, MS3 Data
DescriptionThe functionality of proteins is dependent on their spatial and temporal distributions, neither of which is directly measured by static protein abundance. Here we report a mass spectrometry-based proteomics workflow and data analysis pipeline, named Simultaneous Proteome Localization and Turnover (SPLAT), to concurrently examine the turnover dynamics and subcellular distributions of whole cell proteomes under perturbation. SPLAT builds on prior work in protein turnover measurements and subcellular localization profiling, by combining dynamic stable isotope labeling, differential ultracentrifugation, and kinetic modeling to concurrently measure changes in protein turnover and subcellular localization under perturbation in one experiment. Briefly, dynamic SILAC labeled cell lysates were fractionated with ultracentrifugation, digested using a modified FASP protocol, and multiplexed with TMT-10 plex tags. The pooled sample was then fractionated with RPLC and injected into an Orbitrap Fusion Tribrid mass spectrometer coupled to an LC with electrospray ionization source operated in data dependent acquisition mode. Detailed methods can be found in the submission files.
HostingRepositoryjPOST
AnnounceDate2023-11-08
AnnouncementXMLSubmission_2023-11-08_12:20:36.230.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSean Robinson
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex reporter+balance reagent N-acylated residue; TMT6plex reporter+balance reagent acylated N-terminal; Label:13C(6)15N(2); Label:13C(6)15N(4)
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-11-04 19:03:59ID requested
12023-11-08 12:20:36announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: MS3, AC16, spatial proteomics, spatiotemporal, protein turnover, dynamic SILAC, TMT
Contact List
Dr. Edward Lau
lab head
Sean Robinson
contact affiliationUniversity of Colorado, Anschutz Medical Campus
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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