PXD046664 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis reveals the composition of glutamatergic organelles of auditory inner hair cells |
Description | In the ear, inner hair cells (IHCs) employ sophisticated glutamatergic ribbon synapses with afferent neurons to transmit auditory information to the brain. The presynaptic machinery responsible for neurotransmitter release in IHC synapses includes proteins such as the multi-C2-domain protein otoferlin and the vesicular glutamate transporter 3 (VGluT3). Yet, much of this likely unique molecular machinery remains to be deciphered. The scarcity of material has so far hampered biochemical studies which require large amounts of purified sample. We developed a subcellular fractionation workflow combined with immunoisolation of VGluT3-containing membrane vesicles, allowing for the enrichment of IHC trafficking glutamatergic organelles that are likely dominated by synaptic vesicles (SVs) of IHCs. We have characterized their protein composition in mice before and after hearing onset using mass spectrometry and confocal imaging and provide a fully annotated proteome with hitherto unidentified proteins. Despite the prevalence of IHC marker proteins across IHC maturation, the profiles of trafficking proteins differed markedly before and after hearing onset. Our study provides an inventory of the machinery associated with synaptic vesicle-mediated trafficking and presynaptic activity at IHC ribbon synapses and serves as a foundation for future functional studies. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:28:53.636.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Andreia P. Cepeda |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-11-03 23:23:32 | ID requested | |
1 | 2024-02-09 02:31:17 | announced | |
⏵ 2 | 2024-10-22 06:28:54 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: proteomics, inner hair cells, trafficking, VGluT3,vesicles |
Contact List
Henning Urlaub |
contact affiliation | Bioanalytical Mass Spectrometry Group, Max Planck Institute for Multidisciplinary Sciences, Germany Bioanalytics, Institute for Clinical Chemistry, University Medical Center Goettingen, Germany |
contact email | henning.urlaub@mpinat.mpg.de |
lab head | |
Andreia P. Cepeda |
contact affiliation | Max Planck Institute for Multidisciplinary Sciences |
contact email | andreia.cepeda@mpinat.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD046664
- Label: PRIDE project
- Name: Proteomic analysis reveals the composition of glutamatergic organelles of auditory inner hair cells