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PXD046664

PXD046664 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic analysis reveals the composition of glutamatergic organelles of auditory inner hair cells
DescriptionIn the ear, inner hair cells (IHCs) employ sophisticated glutamatergic ribbon synapses with afferent neurons to transmit auditory information to the brain. The presynaptic machinery responsible for neurotransmitter release in IHC synapses includes proteins such as the multi-C2-domain protein otoferlin and the vesicular glutamate transporter 3 (VGluT3). Yet, much of this likely unique molecular machinery remains to be deciphered. The scarcity of material has so far hampered biochemical studies which require large amounts of purified sample. We developed a subcellular fractionation workflow combined with immunoisolation of VGluT3-containing membrane vesicles, allowing for the enrichment of IHC trafficking glutamatergic organelles that are likely dominated by synaptic vesicles (SVs) of IHCs. We have characterized their protein composition in mice before and after hearing onset using mass spectrometry and confocal imaging and provide a fully annotated proteome with hitherto unidentified proteins. Despite the prevalence of IHC marker proteins across IHC maturation, the profiles of trafficking proteins differed markedly before and after hearing onset. Our study provides an inventory of the machinery associated with synaptic vesicle-mediated trafficking and presynaptic activity at IHC ribbon synapses and serves as a foundation for future functional studies.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:28:53.636.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAndreia P. Cepeda
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-11-03 23:23:32ID requested
12024-02-09 02:31:17announced
22024-10-22 06:28:54announced2024-10-22: Updated project metadata.
Publication List
10.1016/J.MCPRO.2023.100704;
Keyword List
submitter keyword: proteomics, inner hair cells, trafficking, VGluT3,vesicles
Contact List
Henning Urlaub
contact affiliationBioanalytical Mass Spectrometry Group, Max Planck Institute for Multidisciplinary Sciences, Germany Bioanalytics, Institute for Clinical Chemistry, University Medical Center Goettingen, Germany
contact emailhenning.urlaub@mpinat.mpg.de
lab head
Andreia P. Cepeda
contact affiliationMax Planck Institute for Multidisciplinary Sciences
contact emailandreia.cepeda@mpinat.mpg.de
dataset submitter
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Dataset FTP location
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