PXD046645

PXD046645 is an original dataset announced via ProteomeXchange.

Title	Targeted mass spectrometry of Tau preparations purified from Alzheimer’s Disease brains plus either the deubiquitylase USP21 or its activity-dead mutant C221A.
Description	To characterise the abundance of ubiquitylated Tau peptides in the presence of active or inactive variants (wt 196-565 vs. C221A) of the broad spectrum deubiquitylase USP21. Tau peptides are obtained from Tau fibrils purified from post mortem human brains diagnosed with Alzheimer’s. Their detection is facilitated by previous training of the mass spectrometer with in vitro ubiquitylated Tau. The same samples were used to evaluate the percentage of peptides modified with ubiquitin
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:46:04.456.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dorothea Anrather
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitinylated lysine
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-03 07:25:45	ID requested
1	2024-06-18 00:40:57	announced
⏵ 2	2024-10-22 06:46:05	announced	2024-10-22: Updated project metadata.

Ferrari L, Bauer B, Qiu Y, Schuschnig M, Klotz S, Anrather D, Juretschke T, Beli P, Gelpi E, Martens S, Tau fibrils evade autophagy by excessive p62 coating and TAX1BP1 exclusion. Sci Adv, 10(24):eadm8449(2024) [pubmed]

10.1126/sciadv.adm8449;

submitter keyword: Alzheimer’s Disease, (De)ubiquitination/ubiquitylation, neurodegeneration

Sascha Martens
contact affiliation	Dr. Sascha Martens, Max Perutz Labs, University of Vienna, Vienna BioCenter, Vienna, Austria
contact email	sascha.martens@univie.ac.at
lab head
Dorothea Anrather
contact affiliation	Max Perutz Labs, Mass Spectrometry Facility, University of Vienna
contact email	dorothea.anrather@univie.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD046645

PRIDE project URI

• PRIDE
  1. PXD046645
     1. Label: PRIDE project
     2. Name: Targeted mass spectrometry of Tau preparations purified from Alzheimer’s Disease brains plus either the deubiquitylase USP21 or its activity-dead mutant C221A.