PXD046634

PXD046634 is an original dataset announced via ProteomeXchange.

Title	Analysis of conformational changes induced by binding of allosteric nanobodies to a bacterial LRRK2 homologue by CX-MS
Description	As part of an in-depth characterization of nanobodies directed against the Chlorobium tepidum protein CtRoco, a bacterial LRRK2 homologue, chemical crosslinking combined with mass spectrometry (CX-MS) was performed. Two allosteric nanobodies binding to two different epitopes in the LRR and the Roc domain of CtRoco, respectively were analysed using the CID-cleavable crosslinker disuccinimidyl sulfoxide (DSSO).
HostingRepository	PRIDE
AnnounceDate	2024-04-23
AnnouncementXML	Submission_2024-04-23_02:48:07.267.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD046634
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Christian Johannes Gloeckner
SpeciesList	scientific name: Chlorobium tepidum; NCBI TaxID: 1097;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-03 03:11:16	ID requested
⏵ 1	2024-04-23 02:48:07	announced

10.7554/ELIFE.94503;

10.6019/PXD046634;

submitter keyword: LRRK2, DSSO, nanobodies, crosslinking, CtRoco

Christian Johannes Gloeckner
contact affiliation	German Center for Neurodegenerative Diseases (DZNE) Otfried-Müller-Strasse 23 72076 Tübingen Germany
contact email	johannes.gloeckner@dzne.de
lab head
Christian Johannes Gloeckner
contact affiliation	DZNE
contact email	johannes.gloeckner@dzne.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD046634

PRIDE project URI

• PRIDE
  1. PXD046634
     1. Label: PRIDE project
     2. Name: Analysis of conformational changes induced by binding of allosteric nanobodies to a bacterial LRRK2 homologue by CX-MS