PXD046605

PXD046605 is an original dataset announced via ProteomeXchange.

Title	APX2, the ascorbate peroxidase-related protein, regulates the levels of plastocyanin in Chlamydomonas
Description	The function of ascorbate peroxidase-related (APX-R) proteins, present in all green photosynthetic eukaryotes, remains unclear. This study focuses on APX-R from Chlamydomonas reinhardtii, namely ascorbate peroxidase 2 (APX2). We showed that apx2 mutants exhibited a faster oxidation of the PSI primary electron donor, P700, upon sudden light increase and a slower re-reduction rate compared to the wild type, pointing to a limitation of plastocyanin. Spectroscopic, proteomic, and immunoblot analyses confirmed that the phenotype was a result of lower levels of plastocyanin in the apx2 mutants. The redox state of P700 did not differ between wild type and apx2 mutants when the loss of function in plastocyanin was nutritionally complemented by growing apx2 mutants under copper deficiency. In this case, cytochrome c6 functionally replaces plastocyanin, confirming that lower levels of plastocyanin was the primary defect caused by absence of APX2. Overall, the results presented here shed light on an unexpected regulation of plastocyanin level under copper replete conditions, induced by ascorbate peroxidase 2 in Chlamydomonas
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:27:57.846.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD046605
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Didier Vertommen
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-02 03:52:10	ID requested
1	2024-01-29 01:01:42	announced
⏵ 2	2024-10-22 06:27:58	announced	2024-10-22: Updated project metadata.

10.6019/PXD046605;

submitter keyword: Chlamydomonas,APX2, plastocyanin, cytochrome c6, copper-deficiency, P700

Claire Remacle
contact affiliation	C. R., Genetics and Physiology of Microalgae, InBios/Phytosystems research unit, University of Liège, 4000 Liège, Belgium Telephone +3243663812
contact email	c.remacle@uliege.be
lab head
Didier Vertommen
contact affiliation	UCL - de Duve Institute, Brussels Belgium
contact email	didier.vertommen@uclouvain.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046605
     1. Label: PRIDE project
     2. Name: APX2, the ascorbate peroxidase-related protein, regulates the levels of plastocyanin in Chlamydomonas