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PXD046605

PXD046605 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAPX2, the ascorbate peroxidase-related protein, regulates the levels of plastocyanin in Chlamydomonas
DescriptionThe function of ascorbate peroxidase-related (APX-R) proteins, present in all green photosynthetic eukaryotes, remains unclear. This study focuses on APX-R from Chlamydomonas reinhardtii, namely ascorbate peroxidase 2 (APX2). We showed that apx2 mutants exhibited a faster oxidation of the PSI primary electron donor, P700, upon sudden light increase and a slower re-reduction rate compared to the wild type, pointing to a limitation of plastocyanin. Spectroscopic, proteomic, and immunoblot analyses confirmed that the phenotype was a result of lower levels of plastocyanin in the apx2 mutants. The redox state of P700 did not differ between wild type and apx2 mutants when the loss of function in plastocyanin was nutritionally complemented by growing apx2 mutants under copper deficiency. In this case, cytochrome c6 functionally replaces plastocyanin, confirming that lower levels of plastocyanin was the primary defect caused by absence of APX2. Overall, the results presented here shed light on an unexpected regulation of plastocyanin level under copper replete conditions, induced by ascorbate peroxidase 2 in Chlamydomonas
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:27:57.846.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD046605
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterDidier Vertommen
SpeciesList scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-11-02 03:52:10ID requested
12024-01-29 01:01:42announced
22024-10-22 06:27:58announced2024-10-22: Updated project metadata.
Publication List
10.6019/PXD046605;
Keyword List
submitter keyword: Chlamydomonas,APX2, plastocyanin, cytochrome c6, copper-deficiency, P700
Contact List
Claire Remacle
contact affiliationC. R., Genetics and Physiology of Microalgae, InBios/Phytosystems research unit, University of Liège, 4000 Liège, Belgium Telephone +3243663812
contact emailc.remacle@uliege.be
lab head
Didier Vertommen
contact affiliationUCL - de Duve Institute, Brussels Belgium
contact emaildidier.vertommen@uclouvain.be
dataset submitter
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Dataset FTP location
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