PXD046601
PXD046601 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | 20210901_WXY_TMT_2plex_3Rep_Huvec_proteins |
| Description | Cells were harvested with intra-cellular proteins extracted and digested with trypsin. Peptides were then labeled with TMT using a TMT10plex mass tag labeling kit (Thermo Fisher Scientific) per the manufacturer’s instructions. TMT-labeled peptides were then separated by high pH reverse-phase high performance liquid chromatography (HPLC) with C18 columns (Agilent BioTek) and dried in a vacuum centrifuge. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was subsequently performed. All fragments were sequentially dissolved in aqueous solution (0.1% formic acid and 2% acetonitrile), loaded onto a home-made reverse-phase analytical column, and subjected to an EASY-nLC™ 1000 ultraperformance liquid chromatography (UPLC) system (Thermo Fisher Scientific) at a constant flow rate of 400 nL/minutes. For MS settings, the applied electrospray voltage was 2.0 kV and the m/z range was 350 to 1800 for the complete scan. Peptide fragments were quantified with Parallel Reaction Monitoring (PRM). Proteins were identified by Swissprot database and quantified using Proteome Discoverer 2.0. |
| HostingRepository | iProX |
| AnnounceDate | 2023-11-01 |
| AnnouncementXML | Submission_2024-03-06_00:16:17.962.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Ying Wang |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF; ACQUITY UPLC |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-11-01 20:32:08 | ID requested | |
| 1 | 2024-01-28 22:50:35 | announced | |
| ⏵ 2 | 2024-03-06 00:16:19 | announced | 2024-03-06: Update information. |
Publication List
| Chen X, Wang Y, Wang JN, Zhang YC, Zhang YR, Sun RX, Qin B, Dai YX, Zhu HJ, Zhao JX, Zhang WW, Ji JD, Yuan ST, Shen QD, Liu QH, Lactylation-driven FTO targets CDK2 to aggravate microvascular anomalies in diabetic retinopathy. EMBO Mol Med, 16(2):294-318(2024) [pubmed] |
Keyword List
| submitter keyword: Proteomic analyses, HUVECs, FTO |
Contact List
| Xue Chen | |
|---|---|
| contact affiliation | Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Nanjing Medical University |
| contact email | drcx1990@vip.163.com |
| lab head | |
| Ying Wang | |
| contact affiliation | Department of Ophthalmology, The First Affiliated Hospital of Nanjing Medical University, Nanjing Medical University |
| contact email | wangying60131@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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