PXD046591

PXD046591 is an original dataset announced via ProteomeXchange.

Title	Galactan polymerization in mycobacteria requires cell wall components
Description	Galactan polymer is a prominent component of the mycobacterial cell wall core. Its biogenesis starts at the cytoplasmic side of the plasma membrane by a build-up of the linker disaccharide [rhamnosyl (Rha) – N-acetyl-glucosaminyl (GlcNAc) phosphate] on the decaprenyl-phosphate carrier. This decaprenyl-P-P-GlcNAc-Rha intermediate is extended by two bifunctional galactosyl transferases, GlfT1 and GlfT2 and then is translocated to the periplasmic space by an ABC transporter Wzm-Wzt. Cell wall core synthesis is then finalized by the action of an array of arabinosyl transferases, mycolyl transferase and ligases that catalyse an attachment of the arabinogalactan polymer to peptidoglycan through the linker region. Earlier studies proposed that galactan polymerization takes place in a specific region of the mycobacterial cell envelope, the so-called PMf domain (plasma membrane free of cell wall components) or intracellular membrane domain (IMD), which localises to the polar region of mycobacterium based on visualization of the GlfT2 enzyme fused with fluorescent tags. In this work, we examined the activity of the galactan-producing cellular machine in the enzyme fractions (cell envelope and plasma membrane) from Mycobacterium smegmatis in the cell-free system using radioactively labelled substrate (UDP-[14C]-Galactose). We found that despite a high abundance of GlfT2 in both these fractions, galactan is produced only in the reaction mixtures containing the cell envelope fraction. Our findings open the discussion about the regulation of GlfT2 with two plausible alternatives: (i) posttranslational modifications and (ii) interactions with other proteins forming an active complex.
HostingRepository	PRIDE
AnnounceDate	2024-02-26
AnnouncementXML	Submission_2024-02-26_03:05:05.645.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD046591
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Maksym Danchenko
SpeciesList	scientific name: Mycobacterium smegmatis MC2 51; NCBI TaxID: 1445611;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-01 11:15:43	ID requested
1	2024-02-14 22:36:48	announced
⏵ 2	2024-02-26 03:05:06	announced	2024-02-26: Updated project metadata.

10.6019/PXD046591;

Savkov, á K, Danchenko M, Fabianov, á V, Bellov, á J, Benc, ú, rov, á M, Husz, á, r S, Kordul, á, kov, á J, Siv, á, kov, á B, Bar, á, th P, Miku, š, ov, á K, Compartmentalization of galactan biosynthesis in mycobacteria. J Biol Chem, 300(3):105768(2024) [pubmed]

10.1016/j.jbc.2024.105768;

submitter keyword: enzyme fraction, M. smegmatis,cell envelope, GlfT1, proteome

Peter Baráth
contact affiliation	Department of Glycobiology, Institute of Chemistry, Slovak Academy of Sciences
contact email	peter.barath@savba.sk
lab head
Maksym Danchenko
contact affiliation	Plant Science and Biodiversity Center SAS
contact email	maxymdan@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD046591

PRIDE project URI

• PRIDE
  1. PXD046591
     1. Label: PRIDE project
     2. Name: Galactan polymerization in mycobacteria requires cell wall components