PXD046585

PXD046585 is an original dataset announced via ProteomeXchange.

Title	An oscillatory transcription factor complex regulates adaptive changes in macropinocytosis during the growth-to-development transition
Description	Macropinocytosis is an evolutionarily conserved endocytic pathway that mediates non-selective bulk uptake of extracellular fluid. It is the major route by which axenic Dictyostelium cells obtain nutrients and has emerged as a nutrient-scavenging pathway for mammalian cells. How cells adjust macropinocytic activity in various physiological or developmental contexts is an important question that remains largely unanswered. We discovered that, in Dictyostelium cells, the transcription factors Hbx5 and MybG form a functional complex in the nucleus to maintain macropinocytic activity during the growth stage. In contrast, during starvation-induced multicellular development, the transcription factor complex undergoes nucleocytoplasmic shuttling in response to oscillatory cyclic adenosine 3',5'-monophosphate (cAMP) signals, which leads to increased cytoplasmic retention of the complex and progressive downregulation of macropinocytosis. Therefore, by coupling macropinocytosis-related gene expression to the cAMP oscillation system that facilitates long-range cell-cell communication, the dynamic translocation of the Hbx5-MybG complex orchestrates a population-level adjustment of macropinocytic activity to adapt to changing environmental conditions.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:21:47.150.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yazhou Hao
SpeciesList	scientific name: Dictyostelium discoideum (Slime mold); NCBI TaxID: 44689;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-11-01 08:41:44	ID requested
1	2024-01-11 20:40:19	announced
⏵ 2	2024-10-22 06:21:47	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Dictyostelium discoideum, Hbx5, LC-MS, MybG, transcription factor

Huaqing Cai
contact affiliation	Institute of Biophysics, Chinese Academy of Sciences
contact email	huaqingcai@ibp.ac.cn
lab head
Yazhou Hao
contact affiliation	National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China
contact email	haoyazhou14@mails.ucas.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD046585

PRIDE project URI

• PRIDE
  1. PXD046585
     1. Label: PRIDE project
     2. Name: An oscillatory transcription factor complex regulates adaptive changes in macropinocytosis during the growth-to-development transition