PXD046549

PXD046549 is an original dataset announced via ProteomeXchange.

Title	Extracellular vesicules proteomic profile of P. brasiliensis yeasts recovered from granulomatous lesions of C57BL/6 WT mice
Description	C57BL/6 WT mice were infected with 1x10^6 P. brasiliensis yeasts. After 8 weeks and 12 weeks of infection the animals were euthanized and the granulomatous lesions were recovered. Then, the yeasts were recovered and cultivated in a rich medium. After cultivation the yeasts extracellular vesicles were isolated and their proteins extracted, digested with trypsin and analyzed by LC-MS/MS. The data show an induced metabolism by the recovered yeasts, with a higher abundance of proteins related to energy metabolism, lipid metabolism, nucleotide metabolism, amino acid metabolism. Additionally there is a higher abundance of some virulence factor.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:42:07.902.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bruno Borges
SpeciesList	scientific name: Paracoccidioides brasiliensis (strain Pb18); NCBI TaxID: 502780;
ModificationList	monohydroxylated residue; deaminated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-31 16:24:11	ID requested
1	2024-05-23 00:58:22	announced
⏵ 2	2024-10-22 06:42:10	announced	2024-10-22: Updated project metadata.

10.1080/21505594.2024.2329573;

Montanari Borges B, Gama de Santana M, Willian Preite N, de Lima Kaminski V, Trentin G, Almeida F, Vieira Loures F, induce TLR4 and dectin-1 expression in innate cells and promote enhanced Th1/Th17 response. Virulence, 15(1):2329573(2024) [pubmed]

submitter keyword: Paracoccidioides brasiliensis

Extracellular vesicles

Granuloma

Flávio Vieira Loures
contact affiliation	Federal University of São Paulo - Institute of Science and Technology - Applied Immunology Lab
contact email	loures@unifesp.br
lab head
Bruno Borges
contact affiliation	UNIFESP
contact email	brumonborges@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD046549

PRIDE project URI

• PRIDE
  1. PXD046549
     1. Label: PRIDE project
     2. Name: Extracellular vesicules proteomic profile of P. brasiliensis yeasts recovered from granulomatous lesions of C57BL/6 WT mice