PXD046546

PXD046546 is an original dataset announced via ProteomeXchange.

Title	RNA polymerase II acts to promote the organization of chromatin following DNA replication
Description	In mammals, following the passage of the replisome, nucleosomes lose their defined positioning and transcription contributes to their re-organisation. However, whether transcription plays a greater role in the organization of chromatin following DNA replication remains unclear. By performing iPOND-TMT time course experiments in the presents of transcription inhibition, we show that RNAPII promotes the re-association of hundreds of proteins with newly replicated DNA (Data uploaded under PXD046514). To show that this function of RNAPII is specific to newly replicated chromatin, an iPOND-TMT 24hr chase experiment was performed in the presence of transcription inhibition to examine its effect on the protein binding on steady state chromatin.
HostingRepository	PRIDE
AnnounceDate	2024-02-14
AnnouncementXML	Submission_2024-02-14_07:53:29.052.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Susanne Bandau
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-31 13:33:53	ID requested
⏵ 1	2024-02-14 07:53:29	announced

10.1038/s44319-024-00085-x;

Bandau S, Alvarez V, Jiang H, Graff S, Sundaramoorthy R, Gierlinski M, Toman M, Owen-Hughes T, Sidoli S, Lamond A, Alabert C, RNA polymerase II promotes the organization of chromatin following DNA replication. EMBO Rep, 25(3):1387-1414(2024) [pubmed]

submitter keyword: TMT,Proteomics, DNA replications

Constance Alabert
contact affiliation	Molecular Cell and Developmental Biology, School of Life Sciences, University of Dundee, United Kingdom
contact email	c.alabert@dundee.ac.uk
lab head
Susanne Bandau
contact affiliation	University of Dundee
contact email	sbandau@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD046546

PRIDE project URI

• PRIDE
  1. PXD046546
     1. Label: PRIDE project
     2. Name: RNA polymerase II acts to promote the organization of chromatin following DNA replication