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PXD046544

PXD046544 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleArabidopsis thylakoid domains proteome
DescriptionThe thylakoid membrane in land plants is organized into an appressed domain (grana), enriched in photosystem (PS) II, and a non-appressed domain (stroma lamellae) enriched in PSI. This ultrastructure ensures control over excitons spillover from PSII to PSI. The bulky machinery required for the repair and biogenesis of PSII and other proteins of the stacked, appressed domain is located in the non-appressed membranes. Thus, the connecting domain (CD) between grana and stroma lamellae, also known as the grana margins (GM), is the key player in both the structural and functional integrity of the photosynthetic machinery. To elucidate the properties and composition of the GM proteome, we have analyzed the different thylakoid domains isolated from membranes in native conformation as well as from thylakoids artificially unstacked to induce a homogeneous protein composition. We show that the composition of the CD is strictly distinct from that of the highly curved structures (the curvature) present at the periphery of grana discs, which are not physically connected to the stroma lamellae. Furthermore, a detailed map of the composition, distribution and interaction of the protein complexes involved in thylakoid membrane function is provided for the grana, CD, curvature and stroma lamellae domains.
HostingRepositoryPRIDE
AnnounceDate2025-02-11
AnnouncementXMLSubmission_2025-02-11_06:12:50.527.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD046544
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAndrea Trotta
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListcarbamoylated residue; phosphorylated residue; acetylated residue; monohydroxylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-10-31 11:57:44ID requested
12025-02-11 06:12:51announced
Publication List
Trotta A, Gunell S, Bajwa AA, Paakkarinen V, Fujii H, Aro EM, Defining the heterogeneous composition of Arabidopsis thylakoid membrane. Plant J, 121(3):e17259(2025) [pubmed]
10.6019/PXD046544;
10.1111/tpj.17259;
Keyword List
submitter keyword: Arabidopsis thaliana, thylakoid membrane, curvature, grana margins, grana core, stroma lamellae, thylakoid domains, connecting domain
Contact List
Eva-Mari Aro
contact affiliationMolecular Plant Biology, University of Turku
contact emailevaaro@utu.fi
lab head
Andrea Trotta
contact affiliationNational Research Council of Italy (CNR)
contact emailandrea.trotta@cnr.it
dataset submitter
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Dataset FTP location
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