PXD046544 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Arabidopsis thylakoid domains proteome |
Description | The thylakoid membrane in land plants is organized into an appressed domain (grana), enriched in photosystem (PS) II, and a non-appressed domain (stroma lamellae) enriched in PSI. This ultrastructure ensures control over excitons spillover from PSII to PSI. The bulky machinery required for the repair and biogenesis of PSII and other proteins of the stacked, appressed domain is located in the non-appressed membranes. Thus, the connecting domain (CD) between grana and stroma lamellae, also known as the grana margins (GM), is the key player in both the structural and functional integrity of the photosynthetic machinery. To elucidate the properties and composition of the GM proteome, we have analyzed the different thylakoid domains isolated from membranes in native conformation as well as from thylakoids artificially unstacked to induce a homogeneous protein composition. We show that the composition of the CD is strictly distinct from that of the highly curved structures (the curvature) present at the periphery of grana discs, which are not physically connected to the stroma lamellae. Furthermore, a detailed map of the composition, distribution and interaction of the protein complexes involved in thylakoid membrane function is provided for the grana, CD, curvature and stroma lamellae domains. |
HostingRepository | PRIDE |
AnnounceDate | 2025-02-11 |
AnnouncementXML | Submission_2025-02-11_06:12:50.527.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD046544 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Andrea Trotta |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | carbamoylated residue; phosphorylated residue; acetylated residue; monohydroxylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-10-31 11:57:44 | ID requested | |
⏵ 1 | 2025-02-11 06:12:51 | announced | |
Publication List
Trotta A, Gunell S, Bajwa AA, Paakkarinen V, Fujii H, Aro EM, Defining the heterogeneous composition of Arabidopsis thylakoid membrane. Plant J, 121(3):e17259(2025) [pubmed] |
10.6019/PXD046544; |
10.1111/tpj.17259; |
Keyword List
submitter keyword: Arabidopsis thaliana, thylakoid membrane, curvature, grana margins, grana core, stroma lamellae, thylakoid domains, connecting domain |
Contact List
Eva-Mari Aro |
contact affiliation | Molecular Plant Biology, University of Turku |
contact email | evaaro@utu.fi |
lab head | |
Andrea Trotta |
contact affiliation | National Research Council of Italy (CNR) |
contact email | andrea.trotta@cnr.it |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD046544
- Label: PRIDE project
- Name: Arabidopsis thylakoid domains proteome