PXD046544

PXD046544 is an original dataset announced via ProteomeXchange.

Title	Arabidopsis thylakoid domains proteome
Description	The thylakoid membrane in land plants is organized into an appressed domain (grana), enriched in photosystem (PS) II, and a non-appressed domain (stroma lamellae) enriched in PSI. This ultrastructure ensures control over excitons spillover from PSII to PSI. The bulky machinery required for the repair and biogenesis of PSII and other proteins of the stacked, appressed domain is located in the non-appressed membranes. Thus, the connecting domain (CD) between grana and stroma lamellae, also known as the grana margins (GM), is the key player in both the structural and functional integrity of the photosynthetic machinery. To elucidate the properties and composition of the GM proteome, we have analyzed the different thylakoid domains isolated from membranes in native conformation as well as from thylakoids artificially unstacked to induce a homogeneous protein composition. We show that the composition of the CD is strictly distinct from that of the highly curved structures (the curvature) present at the periphery of grana discs, which are not physically connected to the stroma lamellae. Furthermore, a detailed map of the composition, distribution and interaction of the protein complexes involved in thylakoid membrane function is provided for the grana, CD, curvature and stroma lamellae domains.
HostingRepository	PRIDE
AnnounceDate	2025-02-11
AnnouncementXML	Submission_2025-02-11_06:12:50.527.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD046544
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andrea Trotta
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	carbamoylated residue; phosphorylated residue; acetylated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-31 11:57:44	ID requested
⏵ 1	2025-02-11 06:12:51	announced

Trotta A, Gunell S, Bajwa AA, Paakkarinen V, Fujii H, Aro EM, Defining the heterogeneous composition of Arabidopsis thylakoid membrane. Plant J, 121(3):e17259(2025) [pubmed]

10.6019/PXD046544;

10.1111/tpj.17259;

submitter keyword: Arabidopsis thaliana, thylakoid membrane, curvature, grana margins, grana core, stroma lamellae, thylakoid domains, connecting domain

Eva-Mari Aro
contact affiliation	Molecular Plant Biology, University of Turku
contact email	evaaro@utu.fi
lab head
Andrea Trotta
contact affiliation	National Research Council of Italy (CNR)
contact email	andrea.trotta@cnr.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046544
     1. Label: PRIDE project
     2. Name: Arabidopsis thylakoid domains proteome