PXD046534

PXD046534 is an original dataset announced via ProteomeXchange.

Title	Optimized mucin-selective enrichment strategy to probe the mucinome
Description	Mucin-domain glycoproteins are densely O-glycosylated and play critical roles in a host of biological functions. Previously, we developed a mucin-selective enrichment strategy by employing a catalytically inactive mucinase (StcE) conjugated to solid support. While this method was effective, it suffered from low throughput and high sample requirements. Further, we recently introduced a new mucinase (SmE) and demonstrated it has complementary recognition patterns to StcE. Here, we optimized our previous enrichment method to increase throughput and lower sample input amounts while maintaining mucin selectivity and enhancing glycopeptide signal. We also compare the ability of different mucinases, lectins, and mucin binding domains to enrich mucins from various cell lines and human serum. Overall, we demonstrate that our improved method StcE effectively isolates more mucin-domain glycoproteins, resulting in a high number of O-glycopeptides, which will allow for enhanced analysis of the mucinome.
HostingRepository	PRIDE
AnnounceDate	2024-04-10
AnnouncementXML	Submission_2024-04-10_14:57:50.379.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vincent Chang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	N-glycosylated residue; O-glycosyl-L-serine; iodoacetamide derivatized residue; O-glycosyl-L-threonine
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-31 08:19:28	ID requested
⏵ 1	2024-04-10 14:57:50	announced

10.1021/ACS.ANALCHEM.3C05762;

submitter keyword: Mass Spectrometry,Proteomics, Glycoproteomics, Mucins, Enrichment

Vincent Chang
contact affiliation	Yale University Department of Chemistry
contact email	vincent.chang@yale.edu
lab head
Vincent Chang
contact affiliation	Yale University
contact email	vincent.chang@yale.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD046534

PRIDE project URI

• PRIDE
  1. PXD046534
     1. Label: PRIDE project
     2. Name: Optimized mucin-selective enrichment strategy to probe the mucinome