PXD046526

PXD046526 is an original dataset announced via ProteomeXchange.

Title	Ubiquiton – an inducible, linkage-specific polyubiquitylation tool
Description	Here we introduce a set of engineered ubiquitin protein ligases and matching ubiquitin acceptor tags for the rapid, inducible linear (M1-), K48-, or K63-linked polyubiquitylation of proteins in yeast and mammalian cells. By applying the so-called ‘Ubiquiton’ (Ubo) system to proteasomal targeting and the endocytic pathway, we validate this tool for soluble cytoplasmic and nuclear as well as chromatin-associated and integral membrane proteins and demonstrate how it can be used to control the localization and stability of its targets. SILAC-based and label-free quantitative proteomics experiments were performed to verify the linkages of the polyubiquitin chains produced on a model substrate, GFP, in yeast and to identify potential off-target effects of a transgenic E3 system in human cells.
HostingRepository	PRIDE
AnnounceDate	2023-12-15
AnnouncementXML	Submission_2023-12-15_09:36:38.257.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jia-Xuan Chen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	N-ethylmaleimide derivatized cysteine; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-31 03:40:30	ID requested
⏵ 1	2023-12-15 09:36:38	announced

10.1016/J.MOLCEL.2023.11.016;

submitter keyword: ubiquitin protein ligase, yeast, human, linkage type,ubiquitylation, E3

Helle Ulrich
contact affiliation	Institute of Molecular Biology, Mainz, Germany
contact email	H.Ulrich@imb-mainz.de
lab head
Jia-Xuan Chen
contact affiliation	Institute of Molecular Biology
contact email	j.chen@imb-mainz.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046526
     1. Label: PRIDE project
     2. Name: Ubiquiton – an inducible, linkage-specific polyubiquitylation tool