PXD046514

PXD046514 is an original dataset announced via ProteomeXchange.

Title	RNA polymerase II promotes the organization of chromatin following DNA replication
Description	Understanding how chromatin organisation is duplicated on the two daughter strands is a central question in epigenetics. In mammals, following the passage of the replisome, nucleosomes lose their defined positioning and transcription contributes to their re-organisation. However, whether transcription plays a greater role in the organization of chromatin following DNA replication remains unclear. Here we have monitored protein re-association to newly replicated DNA upon inhibition of transcription using iPOND coupled to quantitative mass spectrometry. We show that RNAPII acts to promote the re-association of hundreds of proteins with newly replicated chromatin, including ATP-dependent remodellers, transcription factors, DNA repair factors, and histone methyltransferases.
HostingRepository	PRIDE
AnnounceDate	2024-02-14
AnnouncementXML	Submission_2024-02-14_07:47:55.984.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Susanne Bandau
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-30 15:19:08	ID requested
⏵ 1	2024-02-14 07:47:57	announced

10.1038/s44319-024-00085-x;

Bandau S, Alvarez V, Jiang H, Graff S, Sundaramoorthy R, Gierlinski M, Toman M, Owen-Hughes T, Sidoli S, Lamond A, Alabert C, RNA polymerase II promotes the organization of chromatin following DNA replication. EMBO Rep, 25(3):1387-1414(2024) [pubmed]

submitter keyword: TMT,Proteomics, DNA replications

Constance Alabert
contact affiliation	Molecular Cell and Developmental Biology, School of Life Sciences, University of Dundee, United Kingdom
contact email	c.alabert@dundee.ac.uk
lab head
Susanne Bandau
contact affiliation	University of Dundee
contact email	sbandau@dundee.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046514
     1. Label: PRIDE project
     2. Name: RNA polymerase II promotes the organization of chromatin following DNA replication