PXD046512

PXD046512 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of Tks5 interactome to identify common marker of all types of invadosomes.
Description	Invasion of the extracellular matrix is needed for physiological events such as wound healing but is also required at different stages of the metastatic process. The ability to progress and invade through the extracellular matrix is a characteristic shared by both normal and cancer cells through the formation of structures called invadosomes. These invadosomes are dynamic structures that can adopt different organizations such as rosettes, dots or can be reorganized along the type I collagen into linear invadosomes. In this study, we used the universal marker of invadosomes Tks5 to identify common features in the different organizations. We used Tks5 co-immunoprecipitation coupled with mass spectrometry analysis that allowed us to identify a translation machinery in all types of organization and in particular the protein Eif4b. We showed that Eif4b colocalized with Tks5 in dots, rosettes and linear invadosomes. Thanks to functional tests, we showed for the first time that EIF4B and the translation are involved in the degradation function of all types of invadosomes. Finally, we also identified the presence of an endoplasmic reticulum at the level of rosette that colocalizes with Tks5. In summary, we provided a molecular characterization of the different types of invadosomes, in normal and cancerous cells, thanks to the common marker Tks5. Moreover, we demonstrated for the first time the presence of a translation machinery in linear invadosomes essential to their degradation function and more generally in all types of invadosomes. We also identified the colocalization of endoplasmic reticulum with Tks5 in rosette. The combination of molecular and structural analysis of the different invadosomes organization allowed us to better understand the composition of these invasive structures.
HostingRepository	PRIDE
AnnounceDate	2025-11-26
AnnouncementXML	Submission_2025-11-26_06:08:41.974.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dupuy Jean-William
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-30 15:09:04	ID requested
⏵ 1	2025-11-26 06:08:42	announced

Normand L, Bonnard B, Sala M, Di Tommaso S, Dourthe C, Raymond AA, Dupuy JW, Mercier L, Goetz JG, Moreau V, Henriet E, Saltel F, Tks5 interactome reveals endoplasmic-reticulum-associated translation machinery in invadosomes. FEBS J, 292(23):6400-6419(2025) [pubmed]

10.1111/febs.70196;

submitter keyword: Invadosomes, NIH cells, Tks5, quantitative proteome, Translation

Frederic Saltel
contact affiliation	University of Bordeaux, Inserm, UMR1312, BRIC, BoRdeaux Institute of onCology, 146 Rue Léo Saignat, Bordeaux, F-33076, France.
contact email	frederic.saltem@inserm.fr
lab head
Dupuy Jean-William
contact affiliation	OncoProt plateform, UAR TBMCore CNRS 3427 INSERM US005 University of Bordeaux
contact email	jean-william.dupuy@u-bordeaux.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD046512

PRIDE project URI

• PRIDE
  1. PXD046512
     1. Label: PRIDE project
     2. Name: Proteomic analysis of Tks5 interactome to identify common marker of all types of invadosomes.