PXD046499

PXD046499 is an original dataset announced via ProteomeXchange.

Title	Proteome of Corynebacterium glutamicum at different pH values
Description	Recently, we established Corynebacterium glutamicum as a suitable production host for various bacteriocins including garvicin Q (GarQ). Here, we establish secretion of GarQ by C. glutamicum via the Sec translocon. At neutral pH, the cationic peptide is efficiently adsorbed to the negatively charged envelope of producer bacteria limiting availability of the bacteriocin in culture supernatants. Using a reporter strain and proteomic analyses, we identified HtrA, a protease associated with secretion stress, as another potential factor limiting GarQ production.
HostingRepository	PRIDE
AnnounceDate	2024-01-08
AnnouncementXML	Submission_2024-01-08_08:21:31.813.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Valentin Steier
SpeciesList	scientific name: Corynebacterium glutamicum ATCC 13032; NCBI TaxID: 196627;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-30 05:06:15	ID requested
⏵ 1	2024-01-08 08:21:32	announced

Desiderato CK, Hasenauer KM, Reich SJ, Goldbeck O, Holivololona L, Ovchinnikov KV, Reiter A, Oldiges M, Diep DB, Eikmanns BJ, Riedel CU, Garvicin Q: characterization of biosynthesis and mode of action. Microb Cell Fact, 21(1):236(2022) [pubmed]

10.1186/s12934-022-01952-9;

submitter keyword: Corynebacterium glutamicum, pH

Marco Oldiges
contact affiliation	Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich, Germany
contact email	m.oldiges@fz-juelich.de
lab head
Valentin Steier
contact affiliation	Forschungszentrum Jülich
contact email	v.steier@fz-juelich.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD046499

PRIDE project URI

• PRIDE
  1. PXD046499
     1. Label: PRIDE project
     2. Name: Proteome of Corynebacterium glutamicum at different pH values