PXD046488

PXD046488 is an original dataset announced via ProteomeXchange.

Title	Global Analysis of Lysine Lactylation of Germinated Seeds in Wheat
Description	Protein lactylation is a newly discovered posttranslational modification (PTM) and involved in multiple biological processes both in mammalian cells and rice grains. However, the function of lysine lactylation remains unexplored in wheat. In this study, we performed the first comparative proteomes and lysine lactylomes during seed germination of wheat. In total, 8,000 proteins and 927 lactylated sites in 394 proteins were identified at 0 and 12 hour after imbibition (HAI). Functional enrichment analysis showed that glycolysis and the TCA cycle related proteins were significantly enriched, and more differentially lactylated proteins were enriched in up-regulated lactylated proteins at 12 HAI vs 0 HAI through KEGG pathway and protein domain enrichment analysis compared to down-regulated lactylated proteins. Meanwhile, ten particularly preferred amino acids near lactylation sites were found in the embryos of germinated seeds: AA*KlaT, A***KlaD********A, KlaA**T****K, K******A*Kla, K*Kla********K, KlaA******A, Kla*A, KD****Kla, K********Kla and KlaG. These results supplied a comprehensive profile of lysine lactylation of wheat and indicated that protein lysine lactylation played important functions in several biological processes.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_06:09:39.762.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xianliang Ma
SpeciesList	scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-30 00:57:52	ID requested
⏵ 1	2024-01-26 06:09:40	announced

Zhu J, Guo W, Lan Y, Global Analysis of Lysine Lactylation of Germinated Seeds in Wheat. Int J Mol Sci, 24(22):(2023) [pubmed]

10.3390/ijms242216195;

submitter keyword: Posttranslational modification

lactylome

seed germination

wheat

Weiwei Guo
contact affiliation	College of Agronomy, Qingdao Agricultural University, Qingdao Shandong, China
contact email	guowei0509@126.com
lab head
Xianliang Ma
contact affiliation	Micrometer Biotech
contact email	maxianliangyxz@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD046488

PRIDE project URI

• PRIDE
  1. PXD046488
     1. Label: PRIDE project
     2. Name: Global Analysis of Lysine Lactylation of Germinated Seeds in Wheat