⮝ Full datasets listing

PXD046479

PXD046479 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGas5 RIP-LC MS/MS using PFC lysates from behavioral-trained mice
DescriptionLong noncoding RNAs (lncRNAs) represent a multidimensional class of regulatory molecules that are involved in many aspects of brain function. Emerging evidence indicates that lncRNAs are localized to the synapse; however, a direct role for their activity in this subcellular compartment in memory formation has yet to be demonstrated. Using lncRNA capture-seq, we identified a Gas5 variant that accumulate in the synaptic compartment within the infralimbic prefrontal cortex of adult male C57/Bl6 mice. Gas5 RNA immunoprecipitation followed by mass spectrometry revealed that this Gas5 isoform, in association with the RNA binding proteins G3BP2 and CAPRIN1, regulates the activity-dependent trafficking and clustering of RNA granules. In addition, we found that cell-type-specific, activity-dependent, and synapse-specific knockdown of this Gas5 variant led to impaired fear extinction memory. These findings identify a new mechanism of fear extinction that involves the dynamic interaction between local lncRNA activity and RNA condensates in the synaptic compartment.
HostingRepositoryPRIDE
AnnounceDate2023-11-20
AnnouncementXMLSubmission_2023-11-19_17:45:04.537.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterWei-Siang Liau
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentTripleTOF 6600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-10-29 22:23:55ID requested
12023-11-19 17:45:04announced
Publication List
10.1038/S41467-023-43535-1;
Keyword List
submitter keyword: LC MS/MS, PFC, Mice,Gas5
Contact List
Timothy W. Bredy
contact affiliationQueensland Brain Institute, Bredy Lab, University of Queensland, Australia
contact emailt.bredy@uq.edu.au
lab head
Wei-Siang Liau
contact affiliationUniversity of Queensland
contact emailw.liau@uq.edu.au
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD046479
PRIDE project URI
Repository Record List
[ + ]