PXD046479 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Gas5 RIP-LC MS/MS using PFC lysates from behavioral-trained mice |
Description | Long noncoding RNAs (lncRNAs) represent a multidimensional class of regulatory molecules that are involved in many aspects of brain function. Emerging evidence indicates that lncRNAs are localized to the synapse; however, a direct role for their activity in this subcellular compartment in memory formation has yet to be demonstrated. Using lncRNA capture-seq, we identified a Gas5 variant that accumulate in the synaptic compartment within the infralimbic prefrontal cortex of adult male C57/Bl6 mice. Gas5 RNA immunoprecipitation followed by mass spectrometry revealed that this Gas5 isoform, in association with the RNA binding proteins G3BP2 and CAPRIN1, regulates the activity-dependent trafficking and clustering of RNA granules. In addition, we found that cell-type-specific, activity-dependent, and synapse-specific knockdown of this Gas5 variant led to impaired fear extinction memory. These findings identify a new mechanism of fear extinction that involves the dynamic interaction between local lncRNA activity and RNA condensates in the synaptic compartment. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-20 |
AnnouncementXML | Submission_2023-11-19_17:45:04.537.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Wei-Siang Liau |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | TripleTOF 6600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-10-29 22:23:55 | ID requested | |
⏵ 1 | 2023-11-19 17:45:04 | announced | |
Publication List
Keyword List
submitter keyword: LC MS/MS, PFC, Mice,Gas5 |
Contact List
Timothy W. Bredy |
contact affiliation | Queensland Brain Institute, Bredy Lab, University of Queensland, Australia |
contact email | t.bredy@uq.edu.au |
lab head | |
Wei-Siang Liau |
contact affiliation | University of Queensland |
contact email | w.liau@uq.edu.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD046479 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD046479
- Label: PRIDE project
- Name: Gas5 RIP-LC MS/MS using PFC lysates from behavioral-trained mice