PXD046479

PXD046479 is an original dataset announced via ProteomeXchange.

Title	Gas5 RIP-LC MS/MS using PFC lysates from behavioral-trained mice
Description	Long noncoding RNAs (lncRNAs) represent a multidimensional class of regulatory molecules that are involved in many aspects of brain function. Emerging evidence indicates that lncRNAs are localized to the synapse; however, a direct role for their activity in this subcellular compartment in memory formation has yet to be demonstrated. Using lncRNA capture-seq, we identified a Gas5 variant that accumulate in the synaptic compartment within the infralimbic prefrontal cortex of adult male C57/Bl6 mice. Gas5 RNA immunoprecipitation followed by mass spectrometry revealed that this Gas5 isoform, in association with the RNA binding proteins G3BP2 and CAPRIN1, regulates the activity-dependent trafficking and clustering of RNA granules. In addition, we found that cell-type-specific, activity-dependent, and synapse-specific knockdown of this Gas5 variant led to impaired fear extinction memory. These findings identify a new mechanism of fear extinction that involves the dynamic interaction between local lncRNA activity and RNA condensates in the synaptic compartment.
HostingRepository	PRIDE
AnnounceDate	2023-11-20
AnnouncementXML	Submission_2023-11-19_17:45:04.537.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wei-Siang Liau
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-29 22:23:55	ID requested
⏵ 1	2023-11-19 17:45:04	announced

10.1038/S41467-023-43535-1;

submitter keyword: LC MS/MS, PFC, Mice,Gas5

Timothy W. Bredy
contact affiliation	Queensland Brain Institute, Bredy Lab, University of Queensland, Australia
contact email	t.bredy@uq.edu.au
lab head
Wei-Siang Liau
contact affiliation	University of Queensland
contact email	w.liau@uq.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD046479

PRIDE project URI

• PRIDE
  1. PXD046479
     1. Label: PRIDE project
     2. Name: Gas5 RIP-LC MS/MS using PFC lysates from behavioral-trained mice