PXD046470

PXD046470 is an original dataset announced via ProteomeXchange.

Title	Mechanotransduction coordinates extracellular matrix protein homeostasis promoting longevity in C. elegans
Description	Although it is postulated that dysfunctional extracellular matrices (ECM) drive aging and disease, how ECM integrity assures longevity is unknown. Here, using proteomics and in-vivo monitoring of fluorescently tagged ECM proteins, we systematically examined the ECM composition during Caenorhabditis elegans aging revealing three distinct collagen dynamics. We show that age-dependent stiffening of inert collagen was slowed by longevity interventions through prolonged replenishing of collagens. In genetic and automated lifespan screens for the regulators that drive this remodeling, we identify hemidesmosome-containing structures that span from the exoskeletal ECM through the hypodermis, basement membrane ECM, to the muscles, coupling mechanical forces to adjust ECM gene expression across tissues. The hemidesmosome tension-induced adaptation is mediated via transcriptional co-activator YAP. Our data reveal a novel mechanism of mechano-coupling and synchronizing of two functionally distinct and spatially distant ECMs that is indispensable for longevity. Thus, besides signaling molecules, mechanotransduction-coordinated ECM remodeling systemically promotes healthy aging.
HostingRepository	PRIDE
AnnounceDate	2023-11-16
AnnouncementXML	Submission_2023-11-16_09:11:50.128.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrea Fossati
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	carbamoylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-28 12:39:36	ID requested
⏵ 1	2023-11-16 09:11:50	announced

Dataset with its publication pending

submitter keyword: DIA

Ewald Colin
contact affiliation	ETH Zurich
contact email	collin-ewald@ethz.ch
lab head
Andrea Fossati
contact affiliation	University of California San Francisco, Quantitative Biosciences Institute (QBI), San Francisco, CA, 94158, USA
contact email	andrea.fossati@ucsf.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046470
     1. Label: PRIDE project
     2. Name: Mechanotransduction coordinates extracellular matrix protein homeostasis promoting longevity in C. elegans