PXD046467 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The 15-min Subcellular Proteome |
Description | This project integrated data-independent acquisition (DIA) with capillary electrophoresis electrospray ionization mass spectrometry (CE-ESI-MS) to enable fast single-cell proteomics. With <15 min of effective separation time in a bottom-up proteomics setting, this technology returned ~1,200 proteins from a single HeLa-cell-equivalent proteome amount. Furthermore, using capillary microsampling to collect the proteome from identified cells in a cleavage-stage embryo of the vertebrate Xenopus laevis (frog) embryo, ~1,400 proteins were identified by measuring ~5 ng of the subcellular proteome content aspirated from live Xenopus embryos. CE-ESI-MS with DIA enhanced proteome detection sensitivity and improved analytical throughput. |
HostingRepository | PRIDE |
AnnounceDate | 2024-09-27 |
AnnouncementXML | Submission_2024-09-27_15:46:26.095.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Peter Nemes |
SpeciesList | scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-10-27 18:10:56 | ID requested | |
⏵ 1 | 2024-09-27 15:46:26 | announced | |
Publication List
Keyword List
submitter keyword: blastomere, proteomics, stem cell, Xenopus laevis, mass spectrometry,Data independent acquisition, single cell, capillary electrophoresis |
Contact List
Peter Nemes |
contact affiliation | 1Department of Chemistry & Biochemistry, University of Maryland, College Park, MD 20742 |
contact email | nemes@umd.edu |
lab head | |
Peter Nemes |
contact affiliation | University of Maryland, College Park |
contact email | nemes@umd.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD046467 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD046467
- Label: PRIDE project
- Name: The 15-min Subcellular Proteome