PXD046467

PXD046467 is an original dataset announced via ProteomeXchange.

Title	The 15-min Subcellular Proteome
Description	This project integrated data-independent acquisition (DIA) with capillary electrophoresis electrospray ionization mass spectrometry (CE-ESI-MS) to enable fast single-cell proteomics. With <15 min of effective separation time in a bottom-up proteomics setting, this technology returned ~1,200 proteins from a single HeLa-cell-equivalent proteome amount. Furthermore, using capillary microsampling to collect the proteome from identified cells in a cleavage-stage embryo of the vertebrate Xenopus laevis (frog) embryo, ~1,400 proteins were identified by measuring ~5 ng of the subcellular proteome content aspirated from live Xenopus embryos. CE-ESI-MS with DIA enhanced proteome detection sensitivity and improved analytical throughput.
HostingRepository	PRIDE
AnnounceDate	2024-09-27
AnnouncementXML	Submission_2024-09-27_15:46:26.095.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peter Nemes
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-27 18:10:56	ID requested
⏵ 1	2024-09-27 15:46:26	announced

10.1021/ACS.JPROTEOME.4C00491;

submitter keyword: blastomere, proteomics, stem cell, Xenopus laevis, mass spectrometry,Data independent acquisition, single cell, capillary electrophoresis

Peter Nemes
contact affiliation	1Department of Chemistry & Biochemistry, University of Maryland, College Park, MD 20742
contact email	nemes@umd.edu
lab head
Peter Nemes
contact affiliation	University of Maryland, College Park
contact email	nemes@umd.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD046467

PRIDE project URI

• PRIDE
  1. PXD046467
     1. Label: PRIDE project
     2. Name: The 15-min Subcellular Proteome