PXD046462

PXD046462 is an original dataset announced via ProteomeXchange.

Title	Kinase pathways in cilia disassembly signalling
Description	Aim: To determine the major protein kinase pathways altered upon serum- or LPA-induced cilia disassembly Primary cilia are dynamic structures which must disassemble for a cell to go through mitosis and re-assemble in G0 to transduce essential signals. Also, in non-cycling cells (such as renal epithelial cells), there is evidence to suggest that deregulated cilia disassembly is of critical importance, for example in instances with an excess of a cilia disassembly stimuli (such as Aurora kinase A expression and activation, a key disassembly factor) which is associated with ciliopathy phenotypes and a net loss of ciliated cells. Serum, growth factor or lysophosphatidic acid (LPA) stimulation of ciliated cells are well established experimental paradigms for the induction of cilia disassembly. The signalling network activated by these stimuli that culminates in cilia disassembly is complex and poorly understood. Studies have generally focused on a single or small group of proteins of interest to define their roles in cilia disassembly and the key pathways and kinases activated upon serum stimulation to induce cilia disassembly have not been defined. Moreover, to the best of our knowledge an unbiased analysis of the major pathways activated during cilia disassembly has not been performed. Ciliated hTERT-RPE1 cells were treated with 10% serum or 2 M LPA for 15’ or 2 h to initiate cilia disassembly + pan-class I PI3K inhibitor GDC-0941, followed by label-free phosphoproteomics.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:51:26.531.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Silvia Surinova
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-27 08:46:49	ID requested
1	2024-07-25 10:17:21	announced
⏵ 2	2024-10-22 06:51:26	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: cilia,LC-MS, LFQ, phosphoproteomics

Silvia Surinova
contact affiliation	Team Lead in Proteomics Head of the Proteomics Research Translational Technology Platform UCL Cancer Institute, Paul O’Gorman Building 72 Huntley Street, London, WC1E 6DD, UK
contact email	s.surinova@ucl.ac.uk
lab head
Silvia Surinova
contact affiliation	UCL
contact email	s.surinova@ucl.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046462
     1. Label: PRIDE project
     2. Name: Kinase pathways in cilia disassembly signalling