PXD046460

PXD046460 is an original dataset announced via ProteomeXchange.

Title	Pure and mixed crude extracts of Thauera aromatica K172 and Pseudomonas putida KT2440 for benchmarking GroEL-proteotyping
Description	This dataset has been used to establish GroEL-proteotyping, a targeted proteotyping approach for assessing bacterial community compositions using the taxonomic marker protein GroEL. This dataset contains raw data of two experiments: 1. Pure cultures of T. aromatica (sample names: TA, TB, TC) and P. putida (sample names: PA, PB, PC) were analyzed individually after in-solution digestion 2. T. aromatica and P. putida were cultivated independently and crude extracts were mixed in defined ratios. GroEL was pre-separated by SDS-PAGE (sample names: LOD_..., in-gel digestion)
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:13:26.942.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Simon Klaes
SpeciesList	scientific name: Thauera aromatica K172; NCBI TaxID: 44139; scientific name: Pseudomonas putida KT2440; NCBI TaxID: 160488;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-27 08:31:53	ID requested
1	2023-10-27 08:53:55	announced
⏵ 2	2024-10-22 06:13:27	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: shotgun proteomics, taxonomic profiling, chaperon, sds-page,proteotyping, chaperonin, metaproteomics

Lorenz Adrian
contact affiliation	Helmholtz Centre for Environmental Research - UFZ, Environmental Biotechnology and Technische Universität Berlin, Geobiotechnology
contact email	lorenz.adrian@ufz.de
lab head
Simon Klaes
contact affiliation	Helmholtz Centre for Environmental Research - UFZ
contact email	simon.klaes@ufz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD046460

PRIDE project URI

• PRIDE
  1. PXD046460
     1. Label: PRIDE project
     2. Name: Pure and mixed crude extracts of Thauera aromatica K172 and Pseudomonas putida KT2440 for benchmarking GroEL-proteotyping