PXD046454

PXD046454 is an original dataset announced via ProteomeXchange.

Title	Activity-Based Metaproteomics Driven Discovery and Enzymological Characterization of Novel α-Galactosidases in the Gut Microbiome
Description	The gut microbiota is an essential contributor to human health and disease and offers an extensive resource of enzymes. Although functional metagenomics methods could predict a correlation between enzyme abundance and functional activity, many enzymes in the microbiome still remain uncharacterized. To discover the differing activities between similar annotated proteins in microbiome, approaches capable of detecting biochemical activity with identification of responsible microbes and enzymes are needed. α-Galactosidases (AGALs) are abundant in the host gut microbiota for hydrolysis of galactooligosaccharides, galactose-containing polysaccharides and glycoconjugates, and have multiple biotechnological applications with increasing demand of global AGAL market, such as food ingredients, animal feed, and biomedical sectors. However, many gut microbial AGALs still lack functional biochemical identification, which limits their usage in industrial and therapeutic applications.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:35:02.561.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	huipai Peng
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-27 07:17:45	ID requested
1	2024-04-02 23:52:05	announced
⏵ 2	2024-10-22 06:35:02	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: α-Galactosidases,Mus musculus,Metaproteomics,activity-based probe,Gut Microbiome

Nan Li
contact affiliation	Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China
contact email	nan.li@siat.ac.cn
lab head
huipai Peng
contact affiliation	CAS Key Laboratory for Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences
contact email	hp.peng@siat.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD046454

PRIDE project URI

• PRIDE
  1. PXD046454
     1. Label: PRIDE project
     2. Name: Activity-Based Metaproteomics Driven Discovery and Enzymological Characterization of Novel α-Galactosidases in the Gut Microbiome