PXD046440

PXD046440 is an original dataset announced via ProteomeXchange.

Title	Global organelle profiling reveals subcellular localization and remodeling at proteome scale
Description	Defining the subcellular distribution of all human proteins and its remodeling across cellular states remains a central goal in cell biology. Here, we present a high-resolution strategy to map subcellular organization using organelle immuno-capture coupled to mass spectrometry. We apply this proteomics workflow to a cell-wide collection of membranous and membrane-less compartments. A graph-based representation of our data reveals the subcellular localization of over 7,600 proteins, defines spatial protein networks, and uncovers interconnections between cellular compartments. We demonstrate that our approach can be deployed to comprehensively profile proteome remodeling during cellular perturbation. By characterizing the cellular landscape following hCoV-OC43 viral infection, we discover that many proteins are regulated by changes in their spatial distribution rather than by changes in their total abundance. Our results establish that proteome-wide analysis of subcellular remodeling provides essential insights for the elucidation of cellular responses. Our dataset can be explored at organelles.czbiohub.org.
HostingRepository	PRIDE
AnnounceDate	2023-12-21
AnnouncementXML	Submission_2023-12-20_23:51:47.587.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD046440
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Frank McCarthy
SpeciesList	scientific name: Human coronavirus OC43; NCBI TaxID: 31631; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-27 00:38:25	ID requested
⏵ 1	2023-12-20 23:51:48	announced

10.6019/PXD046440;

10.1101/2023.12.18.572249;

submitter keyword: Human, Infection, OC43, IP-MS

Manuel Leonetti
contact affiliation	Group Leader, Quantitative Cell Science, Chan Zuckerberg Biohub San Francisco, Ca, USA
contact email	manuel.leonetti@czbiohub.org
lab head
Frank McCarthy
contact affiliation	CZ Biohub
contact email	frank.mccarthy@czbiohub.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/12/PXD046440

PRIDE project URI

• PRIDE
  1. PXD046440
     1. Label: PRIDE project
     2. Name: Global organelle profiling reveals subcellular localization and remodeling at proteome scale