PXD046437

PXD046437 is an original dataset announced via ProteomeXchange.

Title	Methanosarcina barkeri TMT proteomics LC-MSMS
Description	Anaerobic methanogens are an important part of the global carbon cycle and energy supply. With industrialization, Fluoranthene (Flu) accumulated in soils, sediments, and sewage plants etc., giving an uncertain impact on methane production of methanogens. Here, the effect of Flu on Methanosarcina barkeri (M. barkeri) were investigated in this paper. The methane yield of strain with 0.01 and 0.1 mg/L Flu was 1.43 and 1.65 times higher than the control. The growth of M. barkeri was also maximized at Flu of 0.1 mg/L with 1.37-fold than control. The physiological features of M. barkeri were enhanced by 0.1 mg/L Flu, such as, cell viability (154%), total-EPS (1.82-fold), and SOD enzyme activity (1.28 times). Notably, TMT proteomics provided a novel insight to reveal that Flu activated the methanogenic metabolic pathway and ABC transporters of M. barkeri, particularly promoted it to synthesize MtrA/C/D/E/G/H (8.2 times total), FrhA/B/G, iron (III)/zinc/molybdenum/vitamin B12 ABC transporter proteins, and the groups of methyltransferase and coenzyme F420 hydrogenase. The upregulated expression of methanogenesis-related enzyme proteins resulted in increased generation and transfer of methane. Overall, this study shed light on the impact of organic pollutants on global carbon cycle of M. barkeri from the molecular biology perspective.
HostingRepository	iProX
AnnounceDate	2023-10-27
AnnouncementXML	Submission_2023-10-26_19:56:29.138.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhiyi Qin
SpeciesList	scientific name: Methanosarcina barkeri MS; NCBI TaxID: 1434108;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-26 19:56:19	ID requested
⏵ 1	2023-10-26 19:56:29	announced

Dataset with its publication pending

submitter keyword: methanogenesis, Methanosarcina barkeri, polycyclic aromatic hydrocarbons, proteomics, global carbon cycle

Yinguang Chen
contact affiliation	Tongji University
contact email	2110527@tongji.edu.cn
lab head
Zhiyi Qin
contact affiliation	Tongji University
contact email	569783383@qq.com
dataset submitter

iProX dataset URI