PXD046434

PXD046434 is an original dataset announced via ProteomeXchange.

Title	The function of acyl-CoA dehydrogenase gene acdA in Mesorhizobium huakuii
Description	Fatty acids β-oxidation (FAO) is the main pathway of fatty acid degradation in organisms. Acyl-CoA dehydrogenase as the first major enzyme in FAO pathways, catalyzes the dehydrogenation reaction of α and β carbon atoms at the site under the action of FAD cofactor. Bioinformatics analysis showed that acdA gene in Mesorhizobium huakuii 7653R encodes the acyl-CoA dehydrogenase. M. huakuii 7653R acdA gene mutant strain and complementary strain were constructed by homologous recombination. mutant HKacdA exhibited delay in growth in AMS minimal medium with NH4Cl as nitrogen source and glucose as carbon source, and showed more sensitivity to H2O2. The function of M. huakuii acdA gene in symbiotic nitrogen fixation was studied through pot experiment of Astragalus sinicus. Mutant HKacdA was severely impaired in its ability to colonize the rhizosphere of A. sinicus. Compared with the plants inoculated with wild-type 7653R, the leaves of HKacdA-inoculated plants appeared were abnormally sparse and yellow, and exhibited obvious symptoms of nitrogen deficiency. Observation of root nodules and bacteroid morphology revealed that the nodules inoculated with HKacdA contained an abnormally thick cortex and bacteroid-containing cells, and some bacteroids accumulated poly-β-hydroxybutyrate (PHB) granules and appeared dissociation and senescent. The mass spectrometry combined with label-free technique was applied to study the differential expression of proteins in bacteroids. A total of 198 differentially expressed proteins were identifified were identifified including 120 up-regulated and 78 down-regulated proteins. Among the differentially expressed proteins, 38 proteins were related to redox，31 proteins were related to amino acid transport, and 11 proteins were connected with nitrogen fixation. Results showed that acdA gene plays a role in rhizobial bacteroid differentiation and nitrogen fixation by affecting amino acid transport, electron transport in nitrogen fixation and nitrogenase activity in nodule bacteroids.
HostingRepository	iProX
AnnounceDate	2023-10-26
AnnouncementXML	Submission_2023-10-26_19:01:07.968.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fuyan Yu
SpeciesList	scientific name: Mesorhizobium huakuii 7653R; NCBI TaxID: 763057;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-26 19:00:57	ID requested
⏵ 1	2023-10-26 19:01:08	announced

Dataset with its publication pending

submitter keyword: Mesorhizobium huakuii 7653R , Acyl-CoA dehydrogenase gene acdA , Redox , Symbiotic nitrogen fixation , Proteomics

Guojun Cheng
contact affiliation	South-Central Minzu University
contact email	chengguojun@mail.scuec.edu.cn
lab head
Fuyan Yu
contact affiliation	South-Central Minzu University
contact email	2445370419@qq.com
dataset submitter

iProX dataset URI