PXD046389 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Biomimetic activation enables direct functionalization with native sugars |
Description | We report a metal- and protecting-group-free strategy that enables the direct anomeric functionalization of unprotected monosaccharides and oligosaccharides in their native forms via glycosyl radical-based cross-coupling with various electrophiles under mild photoirradiation conditions. This approach eliminates the need for pre-installation and removal of protecting groups, solving an enduring problem in the field and providing a general platform to accelerate the preparation of robust carbo-, thio- and selenoglycosyl compounds in high regio- and stereo-selectivity. We also show that the protocol is amenable to the chemical synthesis of unprotected C-glycosylproteins in a post-translational manner that is complementary to the analogous biological O- and N-glycosylation processes. This dataset contains LCMS data of tryptic or LysC digests of PstS, PanC, histone, and SSβG proteins selectively modified by either Mannose, Galactose, or N-Acetylgalactosamine. In total, 7 LCMS files are presented along with the results of the identification by MSFragger, corresponding to the variants of the proteins with different modifications. |
HostingRepository | PRIDE |
AnnounceDate | 2024-08-10 |
AnnouncementXML | Submission_2024-08-10_12:03:20.319.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Nikita Levin |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-10-25 08:21:13 | ID requested | |
⏵ 1 | 2024-08-10 12:03:21 | announced | |
Publication List
10.1038/s41586-024-07548-0; |
Jiang Y, Wei Y, Zhou QY, Sun GQ, Fu XP, Levin N, Zhang Y, Liu WQ, Song N, Mohammed S, Davis BG, Koh MJ, Direct radical functionalization of native sugars. Nature, 631(8020):319-327(2024) [pubmed] |
Keyword List
submitter keyword: peptide mapping, glycosyl radical-based cross-coupling, native sugars, protecting-group-free funxtionalization |
Contact List
Benjamin Guy Davis |
contact affiliation | Rosalind Franklin Institute, Harwell Science and Innovation Campus, Oxford OX11 0FA, UK; Department of Pharmacology, University of Oxford, Oxford OX1 3QT, UK |
contact email | ben.davis@chem.ox.ac.uk |
lab head | |
Nikita Levin |
contact affiliation | Rosalind Franklin Institute |
contact email | nikita.levin@rfi.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD046389 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD046389
- Label: PRIDE project
- Name: Biomimetic activation enables direct functionalization with native sugars