PXD046389

PXD046389 is an original dataset announced via ProteomeXchange.

Title	Biomimetic activation enables direct functionalization with native sugars
Description	We report a metal- and protecting-group-free strategy that enables the direct anomeric functionalization of unprotected monosaccharides and oligosaccharides in their native forms via glycosyl radical-based cross-coupling with various electrophiles under mild photoirradiation conditions. This approach eliminates the need for pre-installation and removal of protecting groups, solving an enduring problem in the field and providing a general platform to accelerate the preparation of robust carbo-, thio- and selenoglycosyl compounds in high regio- and stereo-selectivity. We also show that the protocol is amenable to the chemical synthesis of unprotected C-glycosylproteins in a post-translational manner that is complementary to the analogous biological O- and N-glycosylation processes. This dataset contains LCMS data of tryptic or LysC digests of PstS, PanC, histone, and SSβG proteins selectively modified by either Mannose, Galactose, or N-Acetylgalactosamine. In total, 7 LCMS files are presented along with the results of the identification by MSFragger, corresponding to the variants of the proteins with different modifications.
HostingRepository	PRIDE
AnnounceDate	2024-08-10
AnnouncementXML	Submission_2024-08-10_12:03:20.319.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nikita Levin
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-25 08:21:13	ID requested
⏵ 1	2024-08-10 12:03:21	announced

10.1038/s41586-024-07548-0;

Jiang Y, Wei Y, Zhou QY, Sun GQ, Fu XP, Levin N, Zhang Y, Liu WQ, Song N, Mohammed S, Davis BG, Koh MJ, Direct radical functionalization of native sugars. Nature, 631(8020):319-327(2024) [pubmed]

submitter keyword: peptide mapping, glycosyl radical-based cross-coupling, native sugars, protecting-group-free funxtionalization

Benjamin Guy Davis
contact affiliation	Rosalind Franklin Institute, Harwell Science and Innovation Campus, Oxford OX11 0FA, UK; Department of Pharmacology, University of Oxford, Oxford OX1 3QT, UK
contact email	ben.davis@chem.ox.ac.uk
lab head
Nikita Levin
contact affiliation	Rosalind Franklin Institute
contact email	nikita.levin@rfi.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD046389

PRIDE project URI

• PRIDE
  1. PXD046389
     1. Label: PRIDE project
     2. Name: Biomimetic activation enables direct functionalization with native sugars