PXD046383

PXD046383 is an original dataset announced via ProteomeXchange.

Title	Effects of Elaeagnus latifolia extract on anti-inflammation in LPS-induced macrophages
Description	Inflammation is the essential process for responding to pathogenic infections, cancer, autoimmune diseases, and other inflammatory diseases. Oxidative stress leads to inflammatory responses. This study aims to evaluate whether the polyphenols in the Elaeagnus latifolia (EL) fruit water extract ameliorate inflammation. RAW 264.7 macrophages were stimulated with lipopolysaccharide with or without Elaeagnus latifolia (EL) extract. Quantitative PCR and ELISA were used to examine the gene expression and cytokines. Our results showed that EL extract-treated activated cells significantly decrease ROS and Nitric oxide levels by reducing the p22phox and iNOS expression and lowering cytokine-encoding genes, including IL-6, PG-E2, TNF-α relative to the LPS-activated macrophages. Furthermore, we observed the protein profiling between LPS-stimulated cell with or without EL extract. Our data establish that EL extract has antioxidant and anti-inflammatory activities and provide evidence that EL extract may be a protective compound for inflammatory diseases.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_12:05:26.858.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiradej Makjaroen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-25 06:14:39	ID requested
⏵ 1	2025-05-06 12:05:27	announced

10.3390/antiox13121485;

Munkong N, Jantarach N, Yoysungnoen B, Lonan P, Makjaroen J, Pearngam P, Kumpunya S, Ruxsanawet K, Nanthawong S, Somparn P, Thim-Uam A, Fruit Extract Ameliorates High-Fat Diet-Induced Obesity in Mice and Alleviates Macrophage-Induced Inflammation in Adipocytes In Vitro. Antioxidants (Basel), 13(12):(2024) [pubmed]

submitter keyword: anti-Inflammation, polyphenol, anti-oxidation, macrophages, Elaeagnus latifolia

Arthid Thim-Uam
contact affiliation	Division of Biochemistry, School of Medical Sciences, University of Phayao, Mae Ka 56000, Thailand
contact email	arthidth@gmai.com
lab head
Jiradej Makjaroen
contact affiliation	Center of Excellence in Systems Biology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330
contact email	jiradejmak@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD046383

PRIDE project URI

• PRIDE
  1. PXD046383
     1. Label: PRIDE project
     2. Name: Effects of Elaeagnus latifolia extract on anti-inflammation in LPS-induced macrophages