PXD046381

PXD046381 is an original dataset announced via ProteomeXchange.

Title	Pre-mRNA fate decision by TTP safeguards the fidelity of the inflammatory response
Description	Successful immune responses are dependent on a precisely controlled balance between transcription and mRNA degradation. mRNA decay is driven through RNA-binding proteins (RBP), yet it remains unclear, how and when an individual mRNA molecule is selected for degradation. We investigated this fundamental question by using the anti-inflammatory RBP tristetraprolin (TTP, also known as Zfp36) as a model. Here, we show that TTP determines the fate of its targets concomitantly with their synthesis by binding to the pre-mRNA in the nucleus. Furthermore, we provide evidence that TTP orchestrates the target destabilization via a hierarchical molecular assembly that culminates by the association of mature mRNA with the RNA degradation machinery in the cytoplasm. The early fate decision in the life cycle of a TTP target mRNA prevents the translation of inflammatory mediators, particularly cytokine mRNAs, and promotes efficient cessation of the immune response. Importantly, the TTP homolog ZFP36L1 displays similar characteristics, suggesting a conserved mode of action within the ZFP36 family of RBPs.
HostingRepository	PRIDE
AnnounceDate	2025-01-27
AnnouncementXML	Submission_2025-01-27_01:04:27.702.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	WeiQiang Chen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480; Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-25 03:49:35	ID requested
⏵ 1	2025-01-27 01:04:28	announced

10.1016/J.MOLCEL.2025.01.001;

submitter keyword: mRNA decay, pre-mRNA binding, resolution of inflammation,Inflammation, TTP

Pavel Kovarik
contact affiliation	Max Perutz Labs, Campus Vienna Biocenter 5, 1030 Vienna
contact email	pavel.kovarik@univie.ac.at
lab head
WeiQiang Chen
contact affiliation	Mass Spectrometry Facility, Max Perutz Laboratories Support GmbH
contact email	chenweiqiang@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD046381

PRIDE project URI

• PRIDE
  1. PXD046381
     1. Label: PRIDE project
     2. Name: Pre-mRNA fate decision by TTP safeguards the fidelity of the inflammatory response