PXD046366
PXD046366 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | SARS-CoV-2 nucleocapsid proetin interactome and SUMOylation |
| Description | Viruses, as opportunistic intracellular parasites, hijack the cellular machinery of host cells to support their survival and propagation. Numerous viral proteins are subjected to host-mediated post-translational modifications. Here, we demonstrate that the SARS-CoV-2 nucleocapsid protein (SARS2-NP) is SUMOylated on the lysine 65 residue, which efficiently mediates SARS2-NP’s ability in homo-oligomerization, RNA association, liquid-liquid phase separation (LLPS). Thereby the innate antiviral immune response is suppressed robustly. These roles can be achieved through intermolecular association between SUMO conjugation and a newly identified SUMO-interacting motif in SARS2-NP. Importantly, the widespread SARS2-NP R203K mutation gains a novel site of SUMOylation which further increases SARS2-NP’s LLPS and immunosuppression. Notably, the SUMO E3 ligase TRIM28 is responsible for catalyzing SARS2-NP SUMOylation. An interfering peptide targeting the TRIM28 and SARS2-NP interaction was screened out to block SARS2-NP SUMOylation and LLPS, and consequently inhibit SARS-CoV-2 replication and rescue innate antiviral immunity. Collectively, these data support SARS2-NP SUMOylation is critical for SARS-CoV-2 virulence, and therefore provide a strategy to antagonize SARS-CoV-2. |
| HostingRepository | iProX |
| AnnounceDate | 2023-10-24 |
| AnnouncementXML | Submission_2024-02-18_20:21:43.714.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jiang Ren |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | sumoylated lysine |
| Instrument | LTQ Orbitrap |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-10-24 19:25:15 | ID requested | |
| 1 | 2023-12-07 22:34:35 | announced | |
| ⏵ 2 | 2024-02-18 20:21:44 | announced | 2024-02-19: Update Publication. |
Publication List
| Ren J, Wang S, Zong Z, Pan T, Liu S, Mao W, Huang H, Yan X, Yang B, He X, Zhou F, Zhang L, TRIM28-mediated nucleocapsid protein SUMOylation enhances SARS-CoV-2 virulence. Nat Commun, 15(1):244(2024) [pubmed] |
Keyword List
| submitter keyword: SARS-CoV-2, Nucleocapsid protein, SUMOylation |
Contact List
| Long Zhang | |
|---|---|
| contact affiliation | Zhejiang University |
| contact email | L_Zhang@zju.edu.cn |
| lab head | |
| Jiang Ren | |
| contact affiliation | Zhejiang University |
| contact email | ren-jiang@outlook.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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