PXD046365

PXD046365 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of primary graft dysfunction and high particle flow rate
Description	Background: Primary graft dysfunction (PGD) remains a challenge to lung transplantation (LTx) recipients as a leading cause of poor early outcomes. New methods are needed for the rapid detection of PGD and the measurement of particle flow rate (PFR) from exhaled breath is a novel means to monitor disease. Methods: 22 recipient pigs underwent orthotopic left LTx and were evaluated for PGD on the third post-operative day. Exhaled breath particles (EBPs) and PFR were measured on mechanical ventilation. EBPs were evaluated with mass spectrometry and the proteome was compared to tissue biopsies and bronchoalveolar lavage fluid (BALF). Findings were confirmed in EBPs from 11 human transplant recipients. Results: 9 recipients developed PGD and had significantly higher PFR (686.4 (449.7-8824.0) particles per minute (ppm)) compared to recipients without PGD (116.6 (79.7-307.4) ppm, p=0.0005). From proteomic analysis, porcine and human EBP proteins recapitulated the BAL and adherens and tight junction proteins were underexpressed in PGD tissue. Conclusions: Histological and proteomic analysis found significant changes to the alveolar-capillary barrier to explain the increased PFR in recipients with PGD. Combined with the similarity of proteomic profiles between EBPs and BALF, exhaled breath measurement is proposed as a rapid and non-invasive bedside measurement of PGD.
HostingRepository	PRIDE
AnnounceDate	2024-04-05
AnnouncementXML	Submission_2024-04-05_07:11:30.319.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gabriel Hirdman
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF SCP; timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-24 17:23:37	ID requested
⏵ 1	2024-04-05 07:11:30	announced

10.3389/TI.2024.12298;

submitter keyword: Primary graft dysfunction, lung transplantation, non-invasive monitoring, exhaled breath particles

Sandra Lindstedt
contact affiliation	IKVL, Lund University, Lund, Sweden
contact email	sandra.lindstedt@med.lu.se
lab head
Gabriel Hirdman
contact affiliation	Lund University
contact email	gabriel.hirdman@med.lu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD046365

PRIDE project URI

• PRIDE
  1. PXD046365
     1. Label: PRIDE project
     2. Name: Proteomic analysis of primary graft dysfunction and high particle flow rate