PXD046353

PXD046353 is an original dataset announced via ProteomeXchange.

Title	Deciphering the role of CDK4 in regulating metabolism and cell fate of triple-negative breast cancer cells
Description	The shift on energetic demands of proliferating cells during tumorigenesis requires an intense crosstalk between cell cycle and metabolism. Beyond their role in cell proliferation, cell cycle regulators also modulate intracellular metabolism of normal tissues. Using both genetic and pharmacological approaches, we aimed to determine the metabolic role of CDK4 in TNBC cells. Unexpectedly, deletion of CDK4 only slightly reduces cell proliferation of triple negative breast cancer (TNBC) cell line and allows in vivo tumor formation. Furthermore, pro-apoptotic stimuli fail to induce proper cell death in CDK4-silenced, depleted or long-term CDK4/6 inhibitors-treated TNBC cells, with dampened mitochondrial calcium uptake. In the second mass spectrometry-based experiment linked to this project, we used TMT technology to assay the proteome and phosphoproteome of WT and Cdk4 KO cells triple negative breast cancer cells (MDA-MB-231) subjected to mitophagic stress, i.e. a treatment with Oligomycin + Antimycin A.
HostingRepository	PRIDE
AnnounceDate	2024-11-26
AnnouncementXML	Submission_2024-11-26_01:45:25.039.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manfredo Quadroni
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-24 06:13:46	ID requested
⏵ 1	2024-11-26 01:45:25	announced

10.1101/2024.09.17.613455;

submitter keyword: Mitochondria-ER-contacts, breast cancer, Metabolism, Mitochondria,Cyclin-dependent Kinase 4

Lluis Fajas
contact affiliation	Center for Integrative Genomics, University of Lausanne, Lausanne, Switzerland
contact email	lluis.fajas@unil.ch
lab head
Manfredo Quadroni
contact affiliation	University of Lausanne
contact email	manfredo.quadroni@unil.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046353
     1. Label: PRIDE project
     2. Name: Deciphering the role of CDK4 in regulating metabolism and cell fate of triple-negative breast cancer cells