PXD046347
PXD046347 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | proteomic response of S. aureus with D-3263 treatment |
Description | S. aureus isolate was inoculated and cultured to exponential growth phase (OD600 at 0.5) in TSB and divided into two groups, the control group and the drug treated group. D-3263 was added to the drug treated group until a final concentration of 1/2× MIC and 1/4× MIC reached, and DMSO added in the control group. Then, cultures in both groups were cultivated for 2 h at 200 rpm. The microbial was centrifuged at 12000 rpm for 10 min, washed twice with Phosphate Buffer Saline (PBS). Lyse the cells by bead-beating at 4 °C in RIPA lysis buffer (1% Triton X-100, 1% deoxycholate, 0.1% SDS), The protein reduction, alkylation and tryptic digestion were followed as filter-aided sample preparation (FASP). The digestion products were analyzed with liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS), followed by label-free quantitative analysis. The MS experiment was performed on a Q Exactive plus mass spectrometer. |
HostingRepository | iProX |
AnnounceDate | 2023-10-24 |
AnnouncementXML | Submission_2023-10-24_00:52:35.229.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Xinyi Cao |
SpeciesList | scientific name: Staphylococcus aureus; NCBI TaxID: 1280; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-10-24 00:52:22 | ID requested | |
⏵ 1 | 2023-10-24 00:52:35 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: D-3263, S. aureus |
Contact List
Peiyu Li | |
---|---|
contact affiliation | Nanshan Hospital |
contact email | peiyuli12@fudan.edu.cn |
lab head | |
Xinyi Cao | |
contact affiliation | Nanshan Hospital |
contact email | caoxinyi0120@163.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |