PXD046345
PXD046345 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Formyl-methionine as an early elongational ribosome destabilization signal |
Description | Despite recent findings of cytosolic fMet-protein translation in eukaryotes, the underlying mechanism that limits cytosolic fMet-protein translation remains unknown. Here, we found that fMet-nascent peptide signals premature ribosome dissociation in S. cerevisiae, that we named N-terminal fMet-initiated ribosome quality control (fMet-RQC). pBpa-dependent photo-crosslinking of stalled fMet-nascent peptide co-purified with Nip1 and Arf1/2, suggesting their functional importance in fMet-RQC pathway. Defective fMet-RQC leads to co-translational fMet-protein aggregation hindering cellular stress adaptiveness. We also found that dynamics of fMet-RQC is critical in regulating cytosolic fMet-protein level during extreme environmental stresses, such as prolonged cold stress, for cellular survival. |
HostingRepository | jPOST |
AnnounceDate | 2024-10-24 |
AnnouncementXML | Submission_2024-10-23_08:00:06.986.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD046345 |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | ChangSeok Lee |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; N-formyl-L-proline; L-methionine sulfoxide |
Instrument | Q Exactive Plus; Q Exactive HF-X |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2023-10-23 23:40:43 | ID requested | |
⏵ 1 | 2024-10-23 08:00:07 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: N-terminal formylation, ribosome quality control, translation regulation, stress adaptation |
Contact List
Cheol-Sang Hwang | |
---|---|
lab head | |
ChangSeok Lee | |
contact affiliation | POSTECH |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002352/ |