PXD046344

PXD046344 is an original dataset announced via ProteomeXchange.

Title	SLC4A7 suppresses the oncogenesis of lung adenocarcinoma by reducing lactate transport and protein lactylation
Description	Lactate and protein lactylation play a vital role in tumor pathogenesis. Solute carrier 4A7 (SLC4A7), a crucial transporter, plays a role in cellular acid homeostasis. However, its impact on lactate transport and protein lactylation in solid tumors, especially lung adenocarcinoma (LUAD), remains largely unexplored. In this study, we conducted lactylome analysis and in vitro/in vivo assays to validate the functional regulation mediated by SLC4A7 in LUAD. We observed that SLC4A7 inhibits tumor progression, including metastasis, invasion and proliferation. Mechanistically, SLC4A7 reduces both intracellular and extracellular lactate accumulation and inhibits overall protein lactylation, as confirmed by lactylome analysis. Our findings from analyzing the lactylome revealed that SLC4A7 suppresses lysine lactylation (Kla) of numerous oncogenes as well as glycolysis activity. Additionally, our previous proteomic data focused on CSCs in LUAD indicated low expression levels of SLC4A7 which was further validated using tumor tissue samples from LUAD patients. Moreover, we verified the inhibitory role played by SLC4A7 in regulating tumor stemness. Collectively, our results uncover the inhibitory effect exerted by SLC4A7 on tumor progression through its regulation of lactate transport, protein lcatylation and stemness properties. We propose that targeting SLC4A7 may hold promise as a novel therapeutic strategy for treating LUAD patients.
HostingRepository	iProX
AnnounceDate	2023-10-24
AnnouncementXML	Submission_2023-10-23_22:34:19.438.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Haojie Yan
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-23 22:34:10	ID requested
⏵ 1	2023-10-23 22:34:19	announced

Dataset with its publication pending

submitter keyword: Lung adenocarcinomas, lactate, lactylation, CSCs, SLC4A7

Furong Li
contact affiliation	Shenzhen People’s Hospital, the Second Clinical Medical College of Jinan University; the First Affiliated Hospital, Southern University of Science and Technology
contact email	lifurong@mail.sustech.edu.cn
lab head
Haojie Yan
contact affiliation	Shenzhen People’s Hospital, the Second Clinical Medical College of Jinan University; the First Affiliated Hospital, Southern University of Science and Technology
contact email	yanhaojie1989@126.com
dataset submitter

iProX dataset URI