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PXD046343

PXD046343 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic response of S. aureus with TAK-285 treated
DescriptionS. aureus isolate was inoculated and cultured to exponential growth phase (OD600 at 0.5) in TSB and divided into two groups, the control group and the drug treated group. TAK-285 was added to the drug treated group until a final concentration of 1/2× MIC reached, and DMSO added in the control group. Then, cultures in both groups were cultivated for 2 h at 200 rpm. The microbial was centrifuged at 12000 rpm for 10 min, washed twice with Phosphate Buffer Saline (PBS). Lyse the cells by bead-beating at 4 °C in RIPA lysis buffer (1% Triton X-100, 1% deoxycholate, 0.1% SDS), The protein reduction, alkylation and tryptic digestion were followed as filter-aided sample preparation (FASP). The digestion products were analyzed with liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS), followed by label-free quantitative analysis. The MS experiment was performed on a Q Exactive plus mass spectrometer.
HostingRepositoryiProX
AnnounceDate2023-10-24
AnnouncementXMLSubmission_2023-10-23_22:32:56.989.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJinlian Huang
SpeciesList scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-10-23 22:32:35ID requested
12023-10-23 22:32:57announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: TAK-285 S. aureus
Contact List
Peiyu Li
contact affiliationNanshan Hospital
contact emailpeiyuli12@fudan.edu.cn
lab head
Jinlian Huang
contact affiliationShenzhen University
contact email2110245042@email.szu.edu.cn
dataset submitter
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