PXD046333

PXD046333 is an original dataset announced via ProteomeXchange.

Title	Remodeling of the gastric environment in Helicobacter pylori-induced atrophic gastritis
Description	Helicobacter pylori colonization of the human stomach is a strong risk factor for gastric cancer. To investigate H. pylori-induced gastric molecular alterations, we used a Mongolian gerbil model of gastric carcinogenesis. Histologic evaluation revealed varying levels of atrophic gastritis (a premalignant condition characterized by parietal and chief cell loss) in H. pylori-infected animals, and transcriptional profiling revealed a loss of markers for these cell types. We then assessed the spatial distribution and relative abundance of proteins in the gastric tissues using imaging mass spectrometry and liquid chromatography with tandem mass spectrometry (LC-MS/MS). We detected striking differences in protein content of corpus and antrum tissues. 492 proteins were preferentially localized to the corpus in uninfected animals. The abundance of 91 of these proteins was reduced in H. pylori-infected corpus tissues exhibiting atrophic gastritis compared to infected corpus tissues with non-atrophic gastritis or uninfected corpus tissues; these included numerous proteins with metabolic functions. Fifty proteins localized to the corpus in uninfected animals were diffusely delocalized throughout the stomach in infected tissues with atrophic gastritis; these included numerous proteins with roles in protein processing. Corresponding alterations were not detected in animals infected with a H. pylori ∆cagT mutant (lacking Cag type IV secretion system activity). These results indicate that H. pylori can cause loss of proteins normally localized to the gastric corpus as well as diffuse delocalization of corpus-specific proteins, resulting in marked changes in the normal gastric molecular partitioning into distinct corpus and antrum regions.
HostingRepository	PRIDE
AnnounceDate	2023-12-04
AnnouncementXML	Submission_2023-12-04_12:32:42.747.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hayes McDonald
SpeciesList	scientific name: Meriones unguiculatus; NCBI TaxID: 10047;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-23 12:01:33	ID requested
⏵ 1	2023-12-04 12:32:43	announced

Dataset with its publication pending

submitter keyword: premalignant, proteomics,Helicobacter pylori, inflammation, gastric cancer, biomarkers

Timothy Cover
contact affiliation	Professor of Medicine Professor of Pathology, Microbiology and Immunology Vanderbilt University School of Medicine
contact email	timothy.l.cover@vumc.org
lab head
Hayes McDonald
contact affiliation	Vanderbilt University
contact email	hayes.mcdonald@vanderbilt.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046333
     1. Label: PRIDE project
     2. Name: Remodeling of the gastric environment in Helicobacter pylori-induced atrophic gastritis