PXD046325

PXD046325 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of the physiological E3 ubiquitin ligase responsible for PIN1 degradation
Description	Induced oncoproteins degradation provides an attractive anti-cancer modality. Activation of anaphase-promoting complex (APC/CCDH1) prevents cell cycle entry by targeting crucial mitotic proteins for degradation. Phosphorylation of its co-activator CDH1 modulates the E3 ligase activity, but little is known about its regulation after phosphorylation and how to effectively harness APC/CCDH1 activity to treat cancer. Notably, Proline-directed phosphorylation is regulated by PIN1-catalyzed cis-trans prolyl isomerization to drive tumor malignancy. However, the mechanisms controlling its protein turnover remain elusive. Through proteomic screens, we identify a reciprocal antagonism of PIN1-APC/CCDH1 mediated by domain-oriented phosphorylation-dependent dual interactions as a fundamental mechanism governing mitotic protein stability and cell cycle entry.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:31:54.835.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wenxue Li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-23 07:52:30	ID requested
1	2024-02-28 12:30:53	announced
⏵ 2	2024-10-22 06:31:55	announced	2024-10-22: Updated project metadata.

Ke S, Dang F, Wang L, Chen JY, Naik MT, Thavamani A, Liu Y, Li W, Kim N, Naik NM, Sui H, Tang W, Qiu C, Koikawa K, Batalini F, Wang X, Clohessy JG, Heng YJ, Lahav G, Gray NS, Zho XZ, Wei W, Wulf GM, Lu KP, controls timely G1/S transition and creates therapeutic vulnerability. Res Sq, ():(2023) [pubmed]

10.21203/rs.3.rs-2447544/v1;

submitter keyword: PIN1,Proteomics, CDH1

Yansheng Liu
contact affiliation	Yale Cancer Biology Institute
contact email	yansheng.liu@yale.edu
lab head
Wenxue Li
contact affiliation	Yale University
contact email	wenxue.li@yale.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD046325

PRIDE project URI

• PRIDE
  1. PXD046325
     1. Label: PRIDE project
     2. Name: Proteomic analysis of the physiological E3 ubiquitin ligase responsible for PIN1 degradation