PXD046325 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis of the physiological E3 ubiquitin ligase responsible for PIN1 degradation |
Description | Induced oncoproteins degradation provides an attractive anti-cancer modality. Activation of anaphase-promoting complex (APC/CCDH1) prevents cell cycle entry by targeting crucial mitotic proteins for degradation. Phosphorylation of its co-activator CDH1 modulates the E3 ligase activity, but little is known about its regulation after phosphorylation and how to effectively harness APC/CCDH1 activity to treat cancer. Notably, Proline-directed phosphorylation is regulated by PIN1-catalyzed cis-trans prolyl isomerization to drive tumor malignancy. However, the mechanisms controlling its protein turnover remain elusive. Through proteomic screens, we identify a reciprocal antagonism of PIN1-APC/CCDH1 mediated by domain-oriented phosphorylation-dependent dual interactions as a fundamental mechanism governing mitotic protein stability and cell cycle entry. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:31:54.835.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Wenxue Li |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-10-23 07:52:30 | ID requested | |
1 | 2024-02-28 12:30:53 | announced | |
⏵ 2 | 2024-10-22 06:31:55 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: PIN1,Proteomics, CDH1 |
Contact List
Yansheng Liu |
contact affiliation | Yale Cancer Biology Institute |
contact email | yansheng.liu@yale.edu |
lab head | |
Wenxue Li |
contact affiliation | Yale University |
contact email | wenxue.li@yale.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/02/PXD046325 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD046325
- Label: PRIDE project
- Name: Proteomic analysis of the physiological E3 ubiquitin ligase responsible for PIN1 degradation