PXD046322

PXD046322 is an original dataset announced via ProteomeXchange.

Title	Lipid exchange at ER-trans Golgi contact sites governs polarized cargo sorting
Description	Oxysterol Binding Protein (OSBP) extracts cholesterol from the ER to deliver it to the TGN via the counter exchange and subsequent hydrolysis of the phosphoinositide PI(4)P. Here, we show that this pathway is essential in polarized epithelial cells where it contributes not only to the proper subcellular distribution of cholesterol, but also to the trans-Golgi sorting and trafficking of numerous plasma membrane cargo proteins with apical or basolateral localization. Reducing the expression of OSBP, blocking its activity or inhibiting a PI4Kinase that fuels OSBP with PI(4)P abolishes the epithelial phenotype. Waves of cargo enrichment in the TGN in phase with OSBP and PI(4)P dynamics suggest that OSBP promotes the formation of lipid gradients along the TGN, which help cargo sorting. During their transient passage through the trans-Golgi, polarized plasma membrane proteins get close to OSBP, but fail to be sorted when OSBP is silenced. Thus, OSBP lipid exchange activity is decisive for polarized cargo sorting and distribution in epithelial cells.
HostingRepository	PRIDE
AnnounceDate	2023-11-20
AnnouncementXML	Submission_2023-11-20_08:51:01.885.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD046322
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Kovacs
SpeciesList	scientific name: Canis familiaris (Dog) (Canis lupus familiaris); NCBI TaxID: 9615;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-10-23 06:48:25	ID requested
⏵ 1	2023-11-20 08:51:02	announced

10.1083/JCB.202307051;

10.6019/PXD046322;

submitter keyword: proximity proteomic,OSBP, surface proteomic

Anne-Sophie Gay
contact affiliation	IPMC/CNRS
contact email	gay@ipmc.cnrs.fr
lab head
David Kovacs
contact affiliation	IPMC CNRS
contact email	kovacs@ipmc.cnrs.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD046322
     1. Label: PRIDE project
     2. Name: Lipid exchange at ER-trans Golgi contact sites governs polarized cargo sorting